194636

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4395663..4396160 Replicon chromosome
Accession NZ_CP070906
Organism Escherichia coli strain 88COLEC

Toxin (Protein)


Gene name ghoT Uniprot ID A0A163EQM5
Locus tag JR901_RS20870 Protein ID WP_001672275.1
Coordinates 4395663..4395836 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JR901_RS20875 Protein ID WP_000398619.1
Coordinates 4395864..4396160 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR901_RS20860 4392333..4393790 + 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -
JR901_RS20865 4394027..4395544 + 1518 WP_001295074.1 lysine--tRNA ligase -
JR901_RS20870 4395663..4395836 - 174 WP_001672275.1 type V toxin-antitoxin system toxin GhoT Toxin
JR901_RS20875 4395864..4396160 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JR901_RS20880 4396387..4396659 - 273 WP_000405647.1 N-acetyltransferase -
JR901_RS20885 4396671..4396901 - 231 WP_021557455.1 (4Fe-4S)-binding protein -
JR901_RS20890 4397082..4398713 + 1632 WP_200930391.1 sensor histidine kinase -
JR901_RS20895 4398710..4399429 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4386809..4396160 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T194636 WP_001672275.1 NZ_CP070906:c4395836-4395663 [Escherichia coli]
MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T194636 NZ_CP093912:c1446188-1445913 [Aggregatibacter actinomycetemcomitans]
ATGATTAAGAGTTTTAAACATAAAGGATTACAAGCCTATTTTGAAACCGGTGTAACCAAAGGGATTCAATCTGCACACAA
AAAGAAAATCAACGGTATTCTTGATTTAATTGATAATGCAACTTCTGAAACGGAATTTAAGATGTTTTACCAATGCCATC
CGTTAAAAGGTGACAGAGAAGGAATTTGGTCAATGAAAGTATCGGGTAATTGGCGTATTACTTTTGAGTTTATTAACGGT
GATGCGTATATATTAAACTATGAAGATTATCATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT194636 WP_000398619.1 NZ_CP070906:c4396160-4395864 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT194636 NZ_CP093912:c1445896-1445600 [Aggregatibacter actinomycetemcomitans]
ATGCGTAAACCTGCACATCCGGGCGAAATTTTATTAGACGGTTTTATTGAACCACATGACATTAAGATTAAAGATTTAGC
CGAGCATTTAGGTTTTTCCCGTGAAACGCTTTCGCGGGTCATTCACGGCAAAACGCCAATGACTGCAAATTTAGCGTTGG
CATTGGAGGATGCCGGCATCAGTACCGCCCGTTTATGGCTTTCATTACAAAGCAATTATGATGTTTGGGCATTGAAACAA
ACACGGGAAGCATCCACAATCCGGCCTTTTGTATTCTCGGAACAACGTTTTGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163EQM5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References