Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3828265..3828806 Replicon chromosome
Accession NZ_CP070906
Organism Escherichia coli strain 88COLEC

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag JR901_RS18200 Protein ID WP_000615980.1
Coordinates 3828528..3828806 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag JR901_RS18195 Protein ID WP_000729709.1
Coordinates 3828265..3828525 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR901_RS18180 3824347..3826440 + 2094 WP_021556448.1 flagellar biosynthesis protein FlhA -
JR901_RS18185 3826633..3827130 - 498 WP_021556447.1 REP-associated tyrosine transposase RayT -
JR901_RS18190 3827306..3828055 - 750 WP_000056850.1 C40 family peptidase -
JR901_RS18195 3828265..3828525 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JR901_RS18200 3828528..3828806 + 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JR901_RS18205 3828962..3829702 + 741 WP_001225679.1 murein L,D-transpeptidase -
JR901_RS18210 3829673..3830440 - 768 WP_000333379.1 class II glutamine amidotransferase -
JR901_RS18215 3830646..3831224 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3827306..3837058 9752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T194633 WP_000615980.1 NZ_CP070906:3828528-3828806 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T194633 NZ_CP093912:746299-746577 [Aggregatibacter actinomycetemcomitans]
ATGATTTTATCTTTCAAACATAAGGGACTTGAACAATTTTTTAAAACCGGTTCGACCGCAGGAATTCAAGCCAAACACGC
CAAGAAATTAAATCTGCAATTGGCCACATTAAATAATGCGGAAACGCCTCTTGCTATGAGTGTTCCCAGCTGGAATTTGC
ACAAACTTAAAGGTGATTTGCAAGAGCATTGGGCAGTTACCGTAAACGGAAATTGGCGCTTAACATTTAAATTTGAAAAT
GGTCATGCTGAAGTTGTTGATTATCAGGATTATCATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT194633 WP_000729709.1 NZ_CP070906:3828265-3828525 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT194633 NZ_CP093912:746593-746865 [Aggregatibacter actinomycetemcomitans]
ATGCACAATCCTCCACACCCGGGAGAACTGTTAAAAGAATATATTGATGGCGTGAGTGTCACGAAGGTTGCCCAAAAATT
AGGTGTTTCGCGTGTTACGCTTTCCCGCATTCTTAATGGGAAAGCAAGTATAACGCCTGAAATGGCTGTGCGATTAAGCG
AATTATTGAATACCACAACACCGAAATTATGGCTGGGTATGCAAGCAGACTACGATTTATGGCAAATTGAACAACAACAC
GCCGTATTCAACATCCAACCATTATTTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References