Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1849382..1849884 Replicon chromosome
Accession NZ_CP070906
Organism Escherichia coli strain 88COLEC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JR901_RS08685 Protein ID WP_000767829.1
Coordinates 1849630..1849884 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JR901_RS08680 Protein ID WP_001259255.1
Coordinates 1849382..1849633 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR901_RS08655 1844560..1845627 - 1068 WP_029397756.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JR901_RS08660 1845627..1846697 - 1071 WP_000108963.1 histidinol-phosphate transaminase -
JR901_RS08665 1846694..1847998 - 1305 WP_205899117.1 histidinol dehydrogenase -
JR901_RS08670 1848004..1848903 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
JR901_RS08675 1849049..1849099 - 51 WP_001364200.1 his operon leader peptide -
JR901_RS08680 1849382..1849633 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JR901_RS08685 1849630..1849884 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JR901_RS08690 1849967..1850791 + 825 WP_000754749.1 SDR family oxidoreductase -
JR901_RS08695 1850837..1851766 + 930 WP_165473688.1 LysR family transcriptional regulator -
JR901_RS08700 1851981..1852043 + 63 WP_010723108.1 membrane protein YoeI -
JR901_RS08705 1852033..1853391 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JR901_RS08710 1853570..1854628 + 1059 WP_165473689.1 transport protein YeeE -
JR901_RS08715 1854642..1854869 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T194620 WP_000767829.1 NZ_CP070906:1849630-1849884 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T194620 NZ_CP093911:1441244-1441582 [Aggregatibacter actinomycetemcomitans]
ATGGCGAAAGCTCACAAATGGCCGACGGAATTTCAATATCCCCCAAAATGGCTTGATTCTGCACGCTCCCGTCCAATTTC
ATTATCATTGCCTTTATCCCATAAAGTTTACCGAGAGGATTCGGTTTACAATTTCTTCGATAATCTTTTGCCCGACAATG
AACAAATTCGTTCACGCATTCAGCAACGTTTCCAGACCTCGACCAAATCCCCCTTTGATTTGCTTTCGGCTATTGAGCAG
GATTGCGTTGGGGCGATTCAACTTTATCACGGTGAAAGTCGCTCTGTTCGGCAAATTTATGCGGAGCCGTTAAATGATAA
GAAGTGTAAAACGTGTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT194620 WP_001259255.1 NZ_CP070906:1849382-1849633 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT194620 NZ_CP093911:1440913-1441194 [Aggregatibacter actinomycetemcomitans]
ATGGTTATTACCATCGCAAAAATGCTTTCTCATGTTATTCGGGAATTTCGTTATCAAGGCAACCTCTCACAAACAGATGT
CGCCAAGCTTGCTAGCACCACCCAAGCCAGAATATCGGTTTTTGAAAATGAGCCGGAACGCACGAAATTAGAAACGTTAT
TTAAAATTTTGGCAGGATTAGAGTTAGAACTTATCGTACAACCGCGCAAAAAATCCGCGGTTACGGAAAATTTAATCAGC
GAACCAACACCTGCATTTTCCGATGACGATGAGGCGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References