Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 644023..644822 Replicon chromosome
Accession NZ_CP070906
Organism Escherichia coli strain 88COLEC

Toxin (Protein)


Gene name yhaV Uniprot ID A0A161R7C1
Locus tag JR901_RS03060 Protein ID WP_000347278.1
Coordinates 644023..644487 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JR901_RS03065 Protein ID WP_001307405.1
Coordinates 644487..644822 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR901_RS03030 639035..639469 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JR901_RS03035 639487..640365 - 879 WP_021557244.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JR901_RS03040 640355..641134 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JR901_RS03045 641145..641618 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JR901_RS03050 641641..642910 - 1270 Protein_598 tagatose-bisphosphate aldolase subunit KbaZ -
JR901_RS03055 643159..643968 + 810 WP_000072174.1 aga operon transcriptional regulator AgaR -
JR901_RS03060 644023..644487 - 465 WP_000347278.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JR901_RS03065 644487..644822 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JR901_RS03070 644971..646542 - 1572 WP_001273763.1 galactarate dehydratase -
JR901_RS03075 646917..648251 + 1335 WP_000599633.1 galactarate/glucarate/glycerate transporter GarP -
JR901_RS03080 648267..649037 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17866.28 Da        Isoelectric Point: 9.6924

>T194616 WP_000347278.1 NZ_CP070906:c644487-644023 [Escherichia coli]
MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T194616 NZ_CP093911:c1080252-1079968 [Aggregatibacter actinomycetemcomitans]
ATGAGTTACATCATTGAGTTTATCCCATCATCTGAAAAAGAATTCCGCAAACTCGATATTAATCTGCGTAAACAATTCAT
TGAGAAATTAAAAGAACGCGCCGAAAATCCCCGAGTGTCATCCGCCAAATTACACGGCATGAAAGATTGCTATAAAATCA
AATTACGCAATGTAGGGTATCATTTGGTGTATCAGGTTATTGATGAACGCATTGTGGTCAAAGCCATCTCCGTAGGAAAA
CGCGATCGAAATTTTGTTTATACCAAAGCAAAAAGCAGGCTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT194616 WP_001307405.1 NZ_CP070906:c644822-644487 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT194616 NZ_CP093911:c1080503-1080249 [Aggregatibacter actinomycetemcomitans]
ATGACAAGCCTTGTTTTAACCGATGTTGTCGCCAGTATCACCGAATTAAAAACTAATCCCATGGCGGTACTTCGTGAAGC
CGGTGGAAAACCGCTGGCAATTTTAAACCGCAATGAACCCGCATTTTATTGCGTCCCACCGGCAATTTATGAATATTTGA
TGGAATTAGTCGATGATGCTGAATTGACAAAAATCGTGGAAGAACGTCAACATGAAAAAAGTTATCCTGTTGATTTAGAG
GATTTACTACAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A161R7C1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References