194614

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4267064..4267561 Replicon chromosome
Accession NZ_CP070902
Organism Escherichia coli strain 99COLEC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JR895_RS20530 Protein ID WP_001173343.1
Coordinates 4267064..4267237 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JR895_RS20535 Protein ID WP_000398619.1
Coordinates 4267265..4267561 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR895_RS20520 4263729..4265186 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
JR895_RS20525 4265423..4266940 + 1518 WP_001295074.1 lysine--tRNA ligase -
JR895_RS20530 4267064..4267237 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JR895_RS20535 4267265..4267561 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JR895_RS20540 4267788..4268060 - 273 WP_000405651.1 N-acetyltransferase -
JR895_RS20545 4268072..4268302 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
JR895_RS20550 4268483..4270114 + 1632 WP_001216477.1 sensor histidine kinase -
JR895_RS20555 4270111..4270830 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4258205..4267561 9356


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T194614 WP_001173343.1 NZ_CP070902:c4267237-4267064 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T194614 NZ_CP093910:1828280-1828534 [Aggregatibacter actinomycetemcomitans]
ATGATTTTAGCTTGGACGGAAACCGCCTGGGAAGATTATCTTTATTGGCAGCAAGTTGATAAAAAGACGTTGTTGCGCAT
TAATAAACTTATCCAAAATATTACCAGAGCACCTTTCGAAGGCTTAGGCAATCCTGAACCTTTAAAACATCAGTTATCCG
GGTTTTGGTCTAGAAGAATAGATAAAGAGCATCGTCTTGTATACCAAGTATCGGATAGCCATTTAACGATTATTCAATGC
CGCTATCACTACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT194614 WP_000398619.1 NZ_CP070902:c4267561-4267265 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT194614 NZ_CP093910:1828032-1828283 [Aggregatibacter actinomycetemcomitans]
ATGAATGTAATAAGTTATTCTGCGTTCCGTGCAGAATTAGCAACAACACTTGATCAGGTGGTTGCAGATCATAGCCCAGT
CATGATTACCAGACAAAATGGCAAACACGCTGTTGTAATGAGTTTAGAAGATTTTGCAGCTTACGAAGAAACGGCTTATT
TATTACGTAGTCCAAAAAATAGGGAGCGTTTATTAGCCTCTATTGACCAGCTCAATTCAGGTAAAATCATCGAACGGGAA
CTCCAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References