Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3694585..3695126 Replicon chromosome
Accession NZ_CP070902
Organism Escherichia coli strain 99COLEC

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JR895_RS17805 Protein ID WP_000615976.1
Coordinates 3694848..3695126 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JR895_RS17800 Protein ID WP_000729704.1
Coordinates 3694585..3694845 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR895_RS17780 3690260..3691045 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
JR895_RS17785 3691017..3692729 + 1713 Protein_3473 flagellar biosynthesis protein FlhA -
JR895_RS17790 3692953..3693450 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JR895_RS17795 3693626..3694399 - 774 WP_015675129.1 C40 family peptidase -
JR895_RS17800 3694585..3694845 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JR895_RS17805 3694848..3695126 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JR895_RS17810 3695282..3696022 + 741 WP_001225679.1 murein L,D-transpeptidase -
JR895_RS17815 3695993..3696760 - 768 WP_000333379.1 class II glutamine amidotransferase -
JR895_RS17820 3696966..3697544 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T194613 WP_000615976.1 NZ_CP070902:3694848-3695126 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T194613 NZ_CP093910:1828032-1828283 [Aggregatibacter actinomycetemcomitans]
ATGAATGTAATAAGTTATTCTGCGTTCCGTGCAGAATTAGCAACAACACTTGATCAGGTGGTTGCAGATCATAGCCCAGT
CATGATTACCAGACAAAATGGCAAACACGCTGTTGTAATGAGTTTAGAAGATTTTGCAGCTTACGAAGAAACGGCTTATT
TATTACGTAGTCCAAAAAATAGGGAGCGTTTATTAGCCTCTATTGACCAGCTCAATTCAGGTAAAATCATCGAACGGGAA
CTCCAGGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT194613 WP_000729704.1 NZ_CP070902:3694585-3694845 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT194613 NZ_CP093910:1828280-1828534 [Aggregatibacter actinomycetemcomitans]
ATGATTTTAGCTTGGACGGAAACCGCCTGGGAAGATTATCTTTATTGGCAGCAAGTTGATAAAAAGACGTTGTTGCGCAT
TAATAAACTTATCCAAAATATTACCAGAGCACCTTTCGAAGGCTTAGGCAATCCTGAACCTTTAAAACATCAGTTATCCG
GGTTTTGGTCTAGAAGAATAGATAAAGAGCATCGTCTTGTATACCAAGTATCGGATAGCCATTTAACGATTATTCAATGC
CGCTATCACTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References