Detailed information of TA system TA194612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3688388..3689082
• Replicon: chromosome
• Accession: NZ_CP070902
• Organism: Escherichia coli strain 99COLEC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JR895_RS17765
• Protein ID: WP_001263493.1
• Coordinates: 3688388..3688786 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JR895_RS17770
• Protein ID: WP_000554757.1
• Coordinates: 3688789..3689082 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR895_RS17735	3683388..3684632	-	1245	WP_000189541.1	esterase FrsA	-
-	3684048..3684128	-	81	NuclAT_9	-	-
-	3684048..3684128	-	81	NuclAT_9	-	-
-	3684048..3684128	-	81	NuclAT_9	-	-
-	3684048..3684128	-	81	NuclAT_9	-	-
JR895_RS17740	3684724..3685182	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JR895_RS17745	3685443..3686900	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JR895_RS17750	3686957..3687478	-	522	Protein_3466	peptide chain release factor H	-
JR895_RS17755	3687477..3687680	-	204	Protein_3467	RNA ligase RtcB family protein	-
JR895_RS17760	3687926..3688378	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JR895_RS17765	3688388..3688786	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JR895_RS17770	3688789..3689082	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JR895_RS17775	3689134..3690189	-	1056	WP_001226164.1	DNA polymerase IV	-
JR895_RS17780	3690260..3691045	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
JR895_RS17785	3691017..3692729	+	1713	Protein_3473	flagellar biosynthesis protein FlhA	-
JR895_RS17790	3692953..3693450	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	lfhA	3646950..3694252	47302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T194612 WP_001263493.1 NZ_CP070902:c3688786-3688388 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T194612 NZ_CP093910:1525673-1525957 [Aggregatibacter actinomycetemcomitans]
ATGAGTTACATCATTGAGTTTATCCCATCATCTGAAAAAGAATTCCGCAAACTCGATATTAATCTGCGTAAACAATTCAT
TGAGAAATTAAAAGAACGCGCCGAAAATCCCCGAGTGTCATCCGCCAAATTACACGGCATGAAAGATTGCTATAAAATCA
AATTACGCAATGTAGGGTATCATTTGGTGTATCAGGTTATTGATGAACGCATTGTGGTCAAAGCCATCTCCGTAGGAAAA
CGCGATCGAAATTTTGTTTATACCAAAGCAAAAAGCAGGCTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT194612 WP_000554757.1 NZ_CP070902:c3689082-3688789 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT194612 NZ_CP093910:1525422-1525676 [Aggregatibacter actinomycetemcomitans]
ATGACAAGCCTTGTTTTAACCGATGTTGTCGCCAGTATCACCGAATTAAAAACTAATCCCATGGCGGTACTTCGTGAAGC
CGGTGGAAAACCGCTGGCAATTTTAAACCGCAATGAACCCGCATTTTATTGCGTCCCACCGGCAATTTATGAATATTTGA
TGGAATTAGTCGATGATGCTGAATTGACAAAAATCGTGGAAGAACGTCAACATGAAAAAAGTTATCCTGTTGATTTAGAG
GATTTACTACAATGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6