Detailed information of TA system TA194535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2480646..2481325
• Replicon: chromosome
• Accession: NZ_CP070873
• Organism: Escherichia fergusonii strain EF91

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: JW972_RS12025
• Protein ID: WP_002431667.1
• Coordinates: 2480646..2480948 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JW972_RS12030
• Protein ID: WP_000806441.1
• Coordinates: 2480984..2481325 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW972_RS12000 (2476022)	2476022..2477674	+	1653	WP_181591242.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
JW972_RS12005 (2477712)	2477712..2478215	-	504	WP_223666686.1	hypothetical protein	-
JW972_RS12010 (2478212)	2478212..2478868	-	657	WP_223666687.1	hypothetical protein	-
JW972_RS12015 (2479036)	2479036..2479515	-	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
JW972_RS12020 (2479719)	2479719..2480513	-	795	WP_148048123.1	TraB/GumN family protein	-
JW972_RS12025 (2480646)	2480646..2480948	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JW972_RS12030 (2480984)	2480984..2481325	+	342	WP_000806441.1	HigA family addiction module antitoxin	Antitoxin
JW972_RS12035 (2481439)	2481439..2483943	-	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
JW972_RS12040 (2484250)	2484250..2485539	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T194535 WP_002431667.1 NZ_CP070873:2480646-2480948 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T194535 NZ_CP093548:2671272-2671736 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT194535 WP_000806441.1 NZ_CP070873:2480984-2481325 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT194535 NZ_CP093548:2670937-2671272 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8