Detailed information of TA system TA194518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1732272..1732786
• Replicon: chromosome
• Accession: NZ_CP070858
• Organism: Limosilactobacillus fermentum strain GR1103

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6C1EMT8
• Locus tag: JWS00_RS08865
• Protein ID: WP_035437199.1
• Coordinates: 1732272..1732535 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: JWS00_RS08870
• Protein ID: WP_035437201.1
• Coordinates: 1732532..1732786 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWS00_RS08850	1727748..1728530	+	783	WP_035437193.1	dimethylargininase	-
JWS00_RS08855	1728918..1730315	+	1398	WP_035437196.1	sodium:proton antiporter	-
JWS00_RS08860	1730741..1731862	+	1122	WP_080650628.1	PTS sugar transporter subunit IIC	-
JWS00_RS08865	1732272..1732535	-	264	WP_035437199.1	Txe/YoeB family addiction module toxin	Toxin
JWS00_RS08870	1732532..1732786	-	255	WP_035437201.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JWS00_RS08875	1733035..1733322	+	288	WP_041807836.1	transposase	-
JWS00_RS08880	1733346..1734224	+	879	WP_086031809.1	IS3 family transposase	-
JWS00_RS08885	1735022..1735786	+	765	WP_080650602.1	zinc ribbon domain-containing protein	-
JWS00_RS08890	1735993..1736898	-	906	WP_154244344.1	IS3 family transposase	-
JWS00_RS08895	1736835..1737449	-	615	WP_035431426.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-78)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10252.94 Da
• Isoelectric Point: 10.3349

>T194518 WP_035437199.1 NZ_CP070858:c1732535-1732272 [Limosilactobacillus fermentum]
MSYAIKLKRSANKDLKKIKGSYLEKSFLQIIEQLRKDPLAPNQGFEKLVLPIKGFYSRRINIQHRLVYKVDQDTQTVIIY
SAWSHDE

Nucleotide

• Length: 264 bp

>T194518 NZ_CP093546:c1360538-1359792 [Bacillus sp. K1]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9773.05 Da
• Isoelectric Point: 4.4237

>AT194518 WP_035437201.1 NZ_CP070858:c1732786-1732532 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPVVIKPTRADEKSVVVIGEDDWKAIQETLFLVNHGVDKQIKERENEPLDDFDDV
WGQL

Nucleotide

• Length: 255 bp

>AT194518 NZ_CP093546:c1359792-1359622 [Bacillus sp. K1]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A6C1EMT8