Detailed information of TA system TA194514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 146901..147493
• Replicon: plasmid pPHDD1
• Accession: NZ_CP070626
• Organism: Photobacterium damselae subsp. damselae strain RM-71

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E4WLL0
• Locus tag: A0J47_RS20605
• Protein ID: WP_013404597.1
• Coordinates: 146901..147221 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A0J47_RS20610
• Protein ID: WP_013404598.1
• Coordinates: 147218..147493 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0J47_RS20590	142403..142607	+	205	Protein_133	IS110 family transposase	-
A0J47_RS20595	143779..145131	-	1353	Protein_134	ISNCY family transposase	-
A0J47_RS20600	145880..146614	+	735	WP_081312356.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
A0J47_RS20605	146901..147221	+	321	WP_013404597.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A0J47_RS20610	147218..147493	+	276	WP_013404598.1	helix-turn-helix domain-containing protein	Antitoxin
A0J47_RS20615	147748..147942	+	195	WP_081312355.1	hypothetical protein	-
A0J47_RS20620	147951..149155	+	1205	Protein_139	IS91 family transposase	-
A0J47_RS20625	149311..149907	-	597	WP_005306894.1	DJ-1/PfpI family protein	-
A0J47_RS20630	150736..151947	+	1212	WP_013404602.1	putative manganese transporter	-
A0J47_RS20635	152030..152224	+	195	WP_013404603.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..153429	153429
	-	inside	IScluster/Tn	-	-	144151..149084	4933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12110.34 Da
• Isoelectric Point: 10.0083

>T194514 WP_013404597.1 NZ_CP070626:146901-147221 [Photobacterium damselae subsp. damselae]
MAWKVDFYSGVEDEILAMPPKIQARMIKLLELIELHGANLGPPHTDSMGDGLFEIRAKAREGIGRGLFCYLKGQHVYVLR
AFVKKTNKTPKKEIELARERMKEVKL

Nucleotide

• Length: 321 bp

>T194514 NZ_CP093539:947724-947897 [Staphylococcus hominis]
ATTTTTATGAGTAAAGATAGTAAACGTAATTATCATGAAGACGAAAATGCAATGGTTCAAAATATGAATGATTTAGAAGC
ATTAGGAAAAGAAATGGAACAGATTTCTGAAGAAAATGATGAAGATAAATATAATCAAACACATGATTCAGAAGCGCGTT
CAGATTTAAATTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10061.54 Da
• Isoelectric Point: 8.9869

>AT194514 WP_013404598.1 NZ_CP070626:147218-147493 [Photobacterium damselae subsp. damselae]
MSLSTLKRKALQNPEVKEAYDDLSVEFELINSLISMREKSGLTQDEVAEKMGTKAPNISRLESGRSNPSLKTLVSYAQAC
GFKLNLGFKHA

Nucleotide

• Length: 276 bp

>AT194514 NZ_CP093539:947102-947701 [Staphylococcus hominis]
ATGGCAATGAATTTTAAAGTATTTGAAGATAAACAACATGCAGCAGATTATGCTGGAGATATTATTAGAAAACAATTTAA
CAATAACCCAACTACCATTGCTGGATTCCATTTAAACAAGGATTCTGCACCTGTATTAGATGAACTAAAAAAGTCAGTTG
ATCGTAACGCTGTAGATTTTAGTCAAATTAATATTCTTGATTATGATGATAATCATTCGTTCTATGAAGCTTTAGGTGTA
CCTTCTGAGCAAGTATATTCTATTAGTCTTGATGATGATGCCGAATCATTAATCAATGATAGAATTAAAACAAAAGAAAA
TAAAGGGAAACTGACTTTACAAGTTGTTTCAATCGATAACACTGGACATTTAGATGTTAATATTCGTCAAGGTTTGATGA
AAGCACGTGAAATTATCTTAGTTGTAACTGGTGCTGAAAAAAGTGAAGTTATCAAACGATTATATGAAGAAAATGGTAAA
TCAAACTTTTTACCAGCTGATTTAAAAGTACATCGTATGGTAACAGTTGTTTTAGACAGAGCTGCTGCTGATGGATTACC
AGAAGATGTTAAAGAATACTTCACAGCGAGATTTGCATAA

Structures

Toxin

• AlphaFold DB: E4WLL0