Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1244903..1245500 Replicon chromosome
Accession NZ_CP070602
Organism Klebsiella pneumoniae strain KPA9853

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag JW307_RS07010 Protein ID WP_004142563.1
Coordinates 1245183..1245500 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag JW307_RS07005 Protein ID WP_004142561.1
Coordinates 1244903..1245190 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW307_RS06970 1240207..1240455 + 249 WP_002893037.1 DUF1158 domain-containing protein -
JW307_RS06975 1240473..1240784 - 312 WP_204779915.1 RamA family antibiotic efflux transcriptional regulator -
JW307_RS06980 1240842..1241539 + 698 Protein_1168 IS1 family transposase -
JW307_RS06985 1241622..1242737 - 1116 Protein_1169 MBL fold metallo-hydrolase -
JW307_RS06990 1242916..1243497 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
JW307_RS06995 1243497..1243865 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
JW307_RS07000 1243985..1244638 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
JW307_RS07005 1244903..1245190 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
JW307_RS07010 1245183..1245500 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JW307_RS07015 1245685..1246728 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
JW307_RS07020 1247395..1248261 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
JW307_RS07025 1248370..1249797 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T194489 WP_004142563.1 NZ_CP070602:c1245500-1245183 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T194489 NZ_CP093527:589185-589547 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT194489 WP_004142561.1 NZ_CP070602:c1245190-1244903 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT194489 NZ_CP093527:589018-589188 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References