Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 30331..30988 Replicon plasmid pA4411_IncC
Accession NZ_CP070596
Organism Klebsiella pneumoniae strain KPA4411

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag JW306_RS01695 Protein ID WP_000270043.1
Coordinates 30331..30681 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JW306_RS01700 Protein ID WP_000124640.1
Coordinates 30686..30988 (+) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW306_RS01660 26805..27302 - 498 WP_000062185.1 hypothetical protein -
JW306_RS01665 27305..27793 - 489 WP_001273096.1 DUF1643 domain-containing protein -
JW306_RS01670 27890..28225 + 336 WP_000683476.1 hypothetical protein -
JW306_RS01675 28240..28710 - 471 WP_001281821.1 hypothetical protein -
JW306_RS01680 28703..29074 - 372 WP_000516916.1 hypothetical protein -
JW306_RS01685 29085..29279 - 195 WP_000343597.1 hypothetical protein -
JW306_RS01690 29620..30168 - 549 WP_001061574.1 transcriptional regulator -
JW306_RS01695 30331..30681 + 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JW306_RS01700 30686..30988 + 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
JW306_RS01705 31015..31308 - 294 WP_001239997.1 chromosome segregation protein ParM -
JW306_RS01710 31396..31566 - 171 Protein_36 HU family DNA-binding protein -
JW306_RS01715 31647..32402 - 756 WP_000194037.1 IS21-like element ISAs29 family helper ATPase IstB -
JW306_RS01720 32417..33958 - 1542 WP_041206683.1 IS21 family transposase -
JW306_RS01725 34179..34286 - 108 Protein_39 HU family DNA-binding protein -
JW306_RS01730 34344..34871 - 528 WP_001236377.1 thermonuclease family protein -
JW306_RS01735 35102..35959 - 858 WP_001167032.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aac(6')-Im / aph(2'')-Ib - 1..102008 102008
- flank IS/Tn - - 25309..26013 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-106)

Antitoxin

(33-79)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T194467 WP_000270043.1 NZ_CP070596:30331-30681 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T194467 NZ_CP093495:4017027-4017245 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT194467 WP_000124640.1 NZ_CP070596:30686-30988 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT194467 NZ_CP093495:4016626-4017000 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9


Antitoxin

Source ID Structure

References