Detailed information of TA system TA194464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4823495..4824097
• Replicon: chromosome
• Accession: NZ_CP070593
• Organism: Escherichia coli strain ESC53083

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: JW305_RS24735
• Protein ID: WP_000897302.1
• Coordinates: 4823495..4823806 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JW305_RS24740
• Protein ID: WP_000356397.1
• Coordinates: 4823807..4824097 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW305_RS24710	4819409..4820008	+	600	WP_001296610.1	glucose-1-phosphatase	-
JW305_RS24715	4820002..4820874	+	873	WP_000920762.1	virulence factor BrkB family protein	-
JW305_RS24720	4820871..4821308	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JW305_RS24725	4821353..4822294	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JW305_RS24730	4822358..4823266	-	909	WP_001385591.1	alpha/beta hydrolase	-
JW305_RS24735	4823495..4823806	+	312	WP_000897302.1	hypothetical protein	Toxin
JW305_RS24740	4823807..4824097	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JW305_RS24745	4824456..4824734	+	279	WP_001296612.1	hypothetical protein	-
JW305_RS24750	4825131..4825349	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
JW305_RS24755	4825534..4825983	-	450	WP_032140890.1	hypothetical protein	-
JW305_RS24760	4826299..4827147	-	849	WP_001038650.1	hypothetical protein	-
JW305_RS24765	4827437..4827679	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
JW305_RS24770	4827861..4828790	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T194464 WP_000897302.1 NZ_CP070593:4823495-4823806 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T194464 NZ_CP093495:738948-739433 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT194464 WP_000356397.1 NZ_CP070593:4823807-4824097 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT194464 NZ_CP093495:738658-738951 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: S1P416