Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4012822..4013621 Replicon chromosome
Accession NZ_CP070593
Organism Escherichia coli strain ESC53083

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag JW305_RS20820 Protein ID WP_000347251.1
Coordinates 4013157..4013621 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag JW305_RS20815 Protein ID WP_001296435.1
Coordinates 4012822..4013157 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW305_RS20800 4008607..4009377 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
JW305_RS20805 4009393..4010727 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JW305_RS20810 4011102..4012673 + 1572 WP_001273763.1 galactarate dehydratase -
JW305_RS20815 4012822..4013157 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JW305_RS20820 4013157..4013621 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JW305_RS20825 4013676..4014485 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JW305_RS20830 4014734..4016014 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
JW305_RS20835 4016037..4016510 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JW305_RS20840 4016521..4017300 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JW305_RS20845 4017290..4018168 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JW305_RS20850 4018186..4018620 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T194462 WP_000347251.1 NZ_CP070593:4013157-4013621 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T194462 NZ_CP093495:326786-327154 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT194462 WP_001296435.1 NZ_CP070593:4012822-4013157 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT194462 NZ_CP093495:326568-326789 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References