Detailed information of TA system TA194458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2765388..2765890
• Replicon: chromosome
• Accession: NZ_CP070593
• Organism: Escherichia coli strain ESC53083

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: JW305_RS15095
• Protein ID: WP_000767819.1
• Coordinates: 2765388..2765642 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: JW305_RS15100
• Protein ID: WP_001259253.1
• Coordinates: 2765639..2765890 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW305_RS15070	2761206..2761664	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
JW305_RS15075	2761881..2763239	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
JW305_RS15080	2763229..2763291	-	63	WP_010723108.1	membrane protein YoeI	-
JW305_RS15085	2763506..2764435	-	930	WP_000803366.1	LysR family transcriptional regulator	-
JW305_RS15090	2764481..2765305	-	825	WP_000754737.1	SDR family oxidoreductase	-
JW305_RS15095	2765388..2765642	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
JW305_RS15100	2765639..2765890	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JW305_RS15105	2766173..2766223	+	51	WP_001364200.1	his operon leader peptide	-
JW305_RS15110	2766369..2767268	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
JW305_RS15115	2767274..2768578	+	1305	WP_001296211.1	histidinol dehydrogenase	-
JW305_RS15120	2768575..2769645	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
JW305_RS15125	2769645..2770712	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2761206..2761664	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T194458 WP_000767819.1 NZ_CP070593:c2765642-2765388 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T194458 NZ_CP093489:4680835-4681212 [Klebsiella pneumoniae]
ATGAAAACATTACCCGACACACACGAACGGGAGGCGTCACGCTGCTCGTCTCCTGTGACAATCTGGCAGACACTCCTGGC
CCGGCTGCTGGGCCAGCATTACAGCCTCACGCTGAATGACACGCCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAGTACGCGCTGGTACGTACCGACCAGCCGGGATTC
GGCACCTGTGCCCATTCGCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGTTGACCCGCAG
CAACTACAGAACGGTGAACGACATTATCCGGGGTAAAGATGCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT194458 WP_001259253.1 NZ_CP070593:c2765890-2765639 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT194458 NZ_CP093489:4680411-4680788 [Klebsiella pneumoniae]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAGGGTAACCGGTTGCATTACCTTGCAGATCGCGCAGGTATAAGAGGTCAGT
TCAGCAACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGCTGAAACAACTGGAGTTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
GGGTTACGTTTATATGGCTGTTTATCCGACACCGGCAGCTCCCGCAACAACCGCATAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3