Detailed information of TA system TA194443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1052578..1053257
• Replicon: chromosome
• Accession: NZ_CP070593
• Organism: Escherichia coli strain ESC53083

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: JW305_RS06330
• Protein ID: WP_000057523.1
• Coordinates: 1052578..1052880 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: JW305_RS06335
• Protein ID: WP_000806442.1
• Coordinates: 1052916..1053257 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW305_RS06305	1047954..1049606	+	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
JW305_RS06310	1049644..1050147	-	504	WP_000667000.1	hypothetical protein	-
JW305_RS06315	1050144..1050944	-	801	WP_000439798.1	hypothetical protein	-
JW305_RS06320	1050968..1051447	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
JW305_RS06325	1051651..1052445	-	795	WP_000365147.1	TraB/GumN family protein	-
JW305_RS06330	1052578..1052880	+	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JW305_RS06335	1052916..1053257	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
JW305_RS06340	1053315..1055819	-	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
JW305_RS06345	1056081..1057013	+	933	WP_000883041.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T194443 WP_000057523.1 NZ_CP070593:1052578-1052880 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T194443 NZ_CP093486:3958874-3959092 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT194443 WP_000806442.1 NZ_CP070593:1052916-1053257 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT194443 NZ_CP093486:3958473-3958847 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3