Detailed information of TA system TA194392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 84275..84932
• Replicon: plasmid p52810_IncC
• Accession: NZ_CP070578
• Organism: Klebsiella pneumoniae strain KP52810

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JW303_RS01900
• Protein ID: WP_000270043.1
• Coordinates: 84275..84625 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JW303_RS01905
• Protein ID: WP_000124640.1
• Coordinates: 84630..84932 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW303_RS01865	80749..81246	-	498	WP_000062185.1	hypothetical protein	-
JW303_RS01870	81249..81737	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
JW303_RS01875	81834..82169	+	336	WP_000683476.1	hypothetical protein	-
JW303_RS01880	82184..82654	-	471	WP_001281821.1	hypothetical protein	-
JW303_RS01885	82647..83018	-	372	WP_000516916.1	hypothetical protein	-
JW303_RS01890	83029..83223	-	195	WP_000343597.1	hypothetical protein	-
JW303_RS01895	83564..84112	-	549	WP_001061574.1	transcriptional regulator	-
JW303_RS01900	84275..84625	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JW303_RS01905	84630..84932	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JW303_RS01910	84959..85252	-	294	WP_001239997.1	chromosome segregation protein ParM	-
JW303_RS01915	85340..85510	-	171	Protein_99	HU family DNA-binding protein	-
JW303_RS01920	85591..86346	-	756	WP_000194037.1	IS21-like element ISAs29 family helper ATPase IstB	-
JW303_RS01925	86361..87902	-	1542	WP_041206683.1	IS21 family transposase	-
JW303_RS01930	88123..88230	-	108	Protein_102	HU family DNA-binding protein	-
JW303_RS01935	88288..88815	-	528	WP_001236377.1	thermonuclease family protein	-
JW303_RS01940	89046..89903	-	858	WP_001167032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aac(6')-Im / aph(2'')-Ib	-	1..102009	102009
	-	flank	IS/Tn	-	-	79253..79957	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T194392 WP_000270043.1 NZ_CP070578:84275-84625 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T194392 NZ_CP093465:c3207389-3207287 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT194392 WP_000124640.1 NZ_CP070578:84630-84932 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT194392 NZ_CP093465:3207280-3207425 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9