Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3087264..3087930 Replicon chromosome
Accession NZ_CP070549
Organism Citrobacter freundii strain CF49969

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag JW296_RS17475 Protein ID WP_003847996.1
Coordinates 3087264..3087581 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag JW296_RS17480 Protein ID WP_003837894.1
Coordinates 3087634..3087930 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JW296_RS17460 3082434..3083312 + 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -
JW296_RS17465 3083422..3085140 - 1719 WP_016151099.1 DUF4153 domain-containing protein -
JW296_RS17470 3085519..3087141 + 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
JW296_RS17475 3087264..3087581 + 318 WP_003847996.1 hypothetical protein Toxin
JW296_RS17480 3087634..3087930 + 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
JW296_RS17485 3087987..3089339 - 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
JW296_RS17490 3089336..3090055 - 720 WP_001157751.1 two-component system response regulator OmpR -
JW296_RS17495 3090281..3090754 + 474 WP_003023524.1 transcription elongation factor GreB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T194319 WP_003847996.1 NZ_CP070549:3087264-3087581 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T194319 NZ_CP093407:4221755-4222171 [Salmonella enterica subsp. enterica serovar Agona]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCGGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT194319 WP_003837894.1 NZ_CP070549:3087634-3087930 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT194319 NZ_CP093407:4221528-4221758 [Salmonella enterica subsp. enterica serovar Agona]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References