Detailed information of TA system TA194305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 188590..189247
• Replicon: plasmid p49969_KPC
• Accession: NZ_CP070545
• Organism: Citrobacter freundii strain CF49969

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JW296_RS01130
• Protein ID: WP_000270043.1
• Coordinates: 188590..188940 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JW296_RS01135
• Protein ID: WP_000124640.1
• Coordinates: 188945..189247 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JW296_RS01095	185064..185561	-	498	WP_000062185.1	hypothetical protein	-
JW296_RS01100	185564..186052	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
JW296_RS01105	186149..186484	+	336	WP_000683476.1	hypothetical protein	-
JW296_RS01110	186499..186969	-	471	WP_001281821.1	hypothetical protein	-
JW296_RS01115	186962..187333	-	372	WP_000516916.1	hypothetical protein	-
JW296_RS01120	187344..187538	-	195	WP_000343597.1	hypothetical protein	-
JW296_RS01125	187879..188427	-	549	WP_001061574.1	transcriptional regulator	-
JW296_RS01130	188590..188940	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JW296_RS01135	188945..189247	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JW296_RS01140	189274..189567	-	294	WP_001239997.1	chromosome segregation protein ParM	-
JW296_RS01145	189655..189927	-	273	WP_001043047.1	HU family DNA-binding protein	-
JW296_RS01150	189985..190512	-	528	WP_001236377.1	thermonuclease family protein	-
JW296_RS01155	190743..191600	-	858	WP_001167032.1	hypothetical protein	-
JW296_RS01160	191587..191817	-	231	WP_000972663.1	hypothetical protein	-
JW296_RS01165	191817..192335	-	519	WP_000210756.1	nitrite reductase	-
JW296_RS01170	192332..192778	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
JW296_RS01175	192778..193137	-	360	WP_000422768.1	hypothetical protein	-
JW296_RS01180	193194..193622	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaTEM-1B / aac(3)-IId / aph(2'')-Ib / aac(6')-Im	-	1..269326	269326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T194305 WP_000270043.1 NZ_CP070545:188590-188940 [Citrobacter freundii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T194305 NZ_CP093405:4239289-4239705 [Salmonella enterica subsp. enterica serovar Agona]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCGGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT194305 WP_000124640.1 NZ_CP070545:188945-189247 [Citrobacter freundii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT194305 NZ_CP093405:4239062-4239292 [Salmonella enterica subsp. enterica serovar Agona]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9