Detailed information of TA system TA194149

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 2780007..2780419
• Replicon: chromosome
• Accession: NZ_CP070482
• Organism: Escherichia coli strain BTH101

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: JWV29_RS13555
• Protein ID: WP_000132601.1
• Coordinates: 2780078..2780419 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	2780007..2780083 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWV29_RS13545 (2776870)	2776870..2778459	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
JWV29_RS13550 (2778456)	2778456..2779850	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (2780007)	2780007..2780083	-	77	NuclAT_14	-	Antitoxin
- (2780007)	2780007..2780083	-	77	NuclAT_14	-	Antitoxin
- (2780007)	2780007..2780083	-	77	NuclAT_14	-	Antitoxin
- (2780007)	2780007..2780083	-	77	NuclAT_14	-	Antitoxin
- (2780007)	2780007..2780083	-	77	NuclAT_15	-	Antitoxin
- (2780007)	2780007..2780083	-	77	NuclAT_15	-	Antitoxin
- (2780007)	2780007..2780083	-	77	NuclAT_15	-	Antitoxin
- (2780007)	2780007..2780083	-	77	NuclAT_15	-	Antitoxin
JWV29_RS13555 (2780078)	2780078..2780419	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
JWV29_RS13560 (2780581)	2780581..2781960	+	1380	Protein_2660	5-methylcytosine-specific restriction endonuclease subunit McrB	-
JWV29_RS13565 (2781960)	2781960..2783006	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T194149 WP_000132601.1 NZ_CP070482:2780078-2780419 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T194149 NZ_CP093369:3573799-3574128 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT194149 NZ_CP070482:c2780083-2780007 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin