Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2465567..2466261 Replicon chromosome
Accession NZ_CP070482
Organism Escherichia coli strain BTH101

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JWV29_RS12075 Protein ID WP_001263489.1
Coordinates 2465567..2465965 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JWV29_RS12080 Protein ID WP_000554758.1
Coordinates 2465968..2466261 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2461155) 2461155..2461235 - 81 NuclAT_12 - -
- (2461155) 2461155..2461235 - 81 NuclAT_12 - -
- (2461155) 2461155..2461235 - 81 NuclAT_12 - -
- (2461155) 2461155..2461235 - 81 NuclAT_12 - -
JWV29_RS12050 (2461831) 2461831..2462289 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JWV29_RS12055 (2462550) 2462550..2464007 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JWV29_RS12060 (2464064) 2464064..2464585 - 522 Protein_2369 peptide chain release factor H -
JWV29_RS12065 (2464581) 2464581..2464787 - 207 Protein_2370 RtcB family protein -
JWV29_RS12070 (2465105) 2465105..2465557 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
JWV29_RS12075 (2465567) 2465567..2465965 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JWV29_RS12080 (2465968) 2465968..2466261 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JWV29_RS12085 (2466313) 2466313..2467368 - 1056 WP_001226164.1 DNA polymerase IV -
JWV29_RS12090 (2467439) 2467439..2468224 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JWV29_RS12095 (2468196) 2468196..2469908 + 1713 Protein_2376 flagellar biosynthesis protein FlhA -
JWV29_RS12100 (2470132) 2470132..2470629 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T194147 WP_001263489.1 NZ_CP070482:c2465965-2465567 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T194147 NZ_CP093369:c2911654-2911403 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT194147 WP_000554758.1 NZ_CP070482:c2466261-2465968 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT194147 NZ_CP093369:c2911406-2911152 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References