Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1268027..1268622 Replicon chromosome
Accession NZ_CP070471
Organism Pseudomonas aeruginosa strain B17932

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag JT750_RS05715 Protein ID WP_003113526.1
Coordinates 1268027..1268305 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag JT750_RS05720 Protein ID WP_003111575.1
Coordinates 1268317..1268622 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT750_RS05700 1263452..1264690 + 1239 WP_031686604.1 C69 family dipeptidase -
JT750_RS05705 1264752..1265399 + 648 WP_003095021.1 carbonate dehydratase -
JT750_RS05710 1265470..1267698 - 2229 WP_033991161.1 TonB-dependent receptor -
JT750_RS05715 1268027..1268305 + 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JT750_RS05720 1268317..1268622 + 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
JT750_RS05730 1269028..1270128 - 1101 WP_003099270.1 redox-regulated ATPase YchF -
JT750_RS05735 1270169..1270753 - 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
JT750_RS05740 1270795..1271409 - 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
JT750_RS05745 1271526..1272467 - 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
JT750_RS05755 1272634..1273482 - 849 WP_031686602.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T194124 WP_003113526.1 NZ_CP070471:1268027-1268305 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T194124 NZ_CP093368:c2944012-2943761 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT194124 WP_003111575.1 NZ_CP070471:1268317-1268622 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT194124 NZ_CP093368:c2943764-2943510 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References