Detailed information of TA system TA194121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 23394..24068
• Replicon: plasmid p1B28906
• Accession: NZ_CP070465
• Organism: Klebsiella pneumoniae strain B28906

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: JT748_RS25675
• Protein ID: WP_032720638.1
• Coordinates: 23745..24068 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JT748_RS25670
• Protein ID: WP_032720637.1
• Coordinates: 23394..23696 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT748_RS25630	18587..19159	+	573	WP_044266753.1	hypothetical protein	-
JT748_RS25635	19190..19684	+	495	WP_044266755.1	hypothetical protein	-
JT748_RS25640	19987..20907	+	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
JT748_RS25645	20943..21731	+	789	WP_032720700.1	TSUP family transporter	-
JT748_RS25650	21733..22305	+	573	WP_032720635.1	GNAT family N-acetyltransferase	-
JT748_RS25655	22410..22793	+	384	WP_032720636.1	hypothetical protein	-
JT748_RS25660	22817..22996	+	180	WP_032720701.1	hypothetical protein	-
JT748_RS25665	23184..23351	+	168	WP_153591440.1	hypothetical protein	-
JT748_RS25670	23394..23696	-	303	WP_032720637.1	helix-turn-helix domain-containing protein	Antitoxin
JT748_RS25675	23745..24068	-	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JT748_RS25680	24246..24798	-	553	Protein_26	DUF4113 domain-containing protein	-
JT748_RS25685	25125..25727	-	603	Protein_27	transposase	-
JT748_RS25690	26228..26551	-	324	WP_019725650.1	hypothetical protein	-
JT748_RS25695	26624..27619	-	996	WP_019725651.1	hypothetical protein	-
JT748_RS25700	27916..28566	-	651	WP_015065500.1	SOS response-associated peptidase	-
JT748_RS25705	28613..29011	-	399	Protein_31	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa	-	1..137142	137142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T194121 WP_032720638.1 NZ_CP070465:c24068-23745 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T194121 NZ_CP093368:c2777835-2777732 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT194121 WP_032720637.1 NZ_CP070465:c23696-23394 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT194121 NZ_CP093368:2777694-2777965 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5