Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3883554..3884151 Replicon chromosome
Accession NZ_CP070449
Organism Klebsiella pneumoniae strain B18064

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag JT745_RS18995 Protein ID WP_004142563.1
Coordinates 3883834..3884151 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag JT745_RS18990 Protein ID WP_004142561.1
Coordinates 3883554..3883841 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT745_RS18960 3879649..3879897 + 249 WP_002893037.1 DUF1158 domain-containing protein -
JT745_RS18965 3879915..3880256 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
JT745_RS18970 3880287..3881402 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
JT745_RS18975 3881582..3882166 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
JT745_RS18980 3882163..3882531 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
JT745_RS18985 3882651..3883289 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
JT745_RS18990 3883554..3883841 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
JT745_RS18995 3883834..3884151 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JT745_RS19000 3884336..3885379 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
JT745_RS19005 3886049..3886915 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
JT745_RS19010 3887024..3888451 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T194065 WP_004142563.1 NZ_CP070449:c3884151-3883834 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T194065 NZ_CP093336:c5225791-5225441 [Paenibacillus sp. N3/727]
GTGATCGTAAAACGCGGCGACGTATTTTTTGCGGATCTTTCACCCGTTGTCGGTTCCGAGCAGGGCGGTGTAAGACCGGT
CCTCGTCATTCAGAACGATATCGGTAACCGGTTCAGTCCTACGGTAATTGTTGCGGCTATAACCGCACAAATTCAGAAAG
CTAAACTTCCGACCCACGTTGAAATAGACGCGGCGGCCCATGGATTTGACCGAGATTCCGTAATTTTGCTGGAGCAGATC
CGAACGATTGATAAGCAGCGGTTAACCGATAAGATCACCCATCTGGACGAGGAAACGATGAGCAAGGTGGATGATTCCCT
GCAGATCAGTTTGGGTTTGATTGATTTCTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT194065 WP_004142561.1 NZ_CP070449:c3883841-3883554 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT194065 NZ_CP093336:c5226076-5225795 [Paenibacillus sp. N3/727]
GTGGCCAACATGCAGAACACCAAACGAATCATGATCAGTTTACCGGATCATCTTTTGCAGGAAGTGGACGGGATTGCTCA
GCTGGAAAATTCCAACCGTAGTGAGCTTATCAGGCAGGCCATGAAGCTGTATTTGAATGAACGCAAGAAGCGTTACATTC
GTGAGTCTATGCAGCGGGGTTACATGGAAATGGCTAAGATTAACCTGACTATGGCGTCTGAAGCGTTTCACGCGGAGGAA
GATGCAGACAGCACTCTGGACCGCTTAGTAAGCGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References