Detailed information of TA system TA194001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 356293..356933
• Replicon: chromosome
• Accession: NZ_CP070435
• Organism: Klebsiella pneumoniae strain B5617

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: JT733_RS01650
• Protein ID: WP_016529833.1
• Coordinates: 356293..356640 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JT733_RS01655
• Protein ID: WP_040181955.1
• Coordinates: 356640..356933 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JT733_RS01640	352219..353652	+	1434	WP_002920564.1	glycogen synthase GlgA	-
JT733_RS01645	353670..356117	+	2448	WP_002920561.1	glycogen phosphorylase	-
JT733_RS01650	356293..356640	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JT733_RS01655	356640..356933	+	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
JT733_RS01660	357003..358511	-	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
JT733_RS01665	358716..359045	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
JT733_RS01670	359096..359926	+	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
JT733_RS01675	359976..360734	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T194001 WP_016529833.1 NZ_CP070435:356293-356640 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T194001 NZ_CP093321:4398305-4398586 [Pseudomonas syringae CC440]
ATGCTAGTTGAGTGGCGGCCGGCAGCACGAATGAACCTGAAGCAAATACTTTCCTATATTGCAGACAGAACCGTACGAGC
AACCAGCGAGTTGGGAATGGCAATAGAAGTAGCCACTTCAGCCCTACCTCAACATCCTTACTTGTATCGCCATGGGCTAG
CGCCTGGAACACGGGAAATCGTCGTGCACCCTAATTATCTCGTTGCTTACAAAGTGACAGATCGGATCGAGGTTCTAGCG
GTGCTTCACGCCAGGCAAGCCTACCCTACCGACGCTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT194001 WP_040181955.1 NZ_CP070435:356640-356933 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT194001 NZ_CP093321:4398124-4398315 [Pseudomonas syringae CC440]
ATGAGCGCTCGAATTTCTCCAATCGTGTCGGAGTTTGAAACCGAGGAACAGGCCGCCAGCTACGACAAGTGGTTTCGTGC
GCAGGTCCAGGCGTCAATCAATGATCCGGCACCCAATATTTCACACGATCAGGTCATGGCCGAGATGCGTGCGCTTCTAG
AGTCTAAGCAGCCCCCGTCAGATGCTAGTTGA

Structures

Toxin

• AlphaFold DB: W9BBY1