Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 330104..330690 Replicon chromosome
Accession NZ_CP070435
Organism Klebsiella pneumoniae strain B5617

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag JT733_RS01540 Protein ID WP_023285605.1
Coordinates 330322..330690 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag JT733_RS01535 Protein ID WP_004174006.1
Coordinates 330104..330325 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT733_RS01515 326261..327187 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JT733_RS01520 327184..328461 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
JT733_RS01525 328458..329225 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
JT733_RS01530 329227..329940 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
JT733_RS01535 330104..330325 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JT733_RS01540 330322..330690 + 369 WP_023285605.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JT733_RS01545 330963..332279 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
JT733_RS01550 332386..333273 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
JT733_RS01555 333270..334115 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
JT733_RS01560 334117..335187 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327184..335924 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13534.89 Da        Isoelectric Point: 8.6410

>T194000 WP_023285605.1 NZ_CP070435:330322-330690 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGITVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T194000 NZ_CP093321:c2034010-2033726 [Pseudomonas syringae CC440]
ATGCAGGTTGAGTGGCTCAAAACAGCACTAAAGAATCTGGATGAGGAGGCTGCGTATATCGCACTGGACAATCCAGCTGC
TGCCGCTGCTTTCGTGAAAGCCATTCAATCAAGCGTAACGCAGCTCGCGAGCTTTCCCGCCATGGGCCGGGAAGGACGGA
TTGCCGGAACCAGAGAATGGCCTCTGCCAGACCTGCCTTACCTGATTCCATATCGCATCCGTAGCGGCCGACTACAGGTT
CTACGGATTTTCCACACCAGAAGACAGTCGCCACCCGTGTGGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT194000 WP_004174006.1 NZ_CP070435:330104-330325 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT194000 NZ_CP093321:c2034239-2034000 [Pseudomonas syringae CC440]
ATGTCCACAATGTCTCTACGTCTGCCGGACGAAATGGCAGATACCCTTGCACACCTTGCAAAGGCGACCGGGCGCAGCAA
GTCATTTTTGGCCCTCGACGCGCTCCGCGAATACCTGACACGCGAGGCTTGGCAGATTGCCGAAATTCAGCGTGCCATCG
AAGAAGCCGACGCCGGCGAGTTCGCAAGCCAAGAAGACGTAAAAGCTGTCATGGACAAATGGTCAGGCAATGCAGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References