Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3882209..3882806 Replicon chromosome
Accession NZ_CP070430
Organism Klebsiella pneumoniae strain B3923

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag JT729_RS18990 Protein ID WP_004142563.1
Coordinates 3882489..3882806 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag JT729_RS18985 Protein ID WP_004142561.1
Coordinates 3882209..3882496 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT729_RS18955 3878304..3878552 + 249 WP_002893037.1 DUF1158 domain-containing protein -
JT729_RS18960 3878570..3878911 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
JT729_RS18965 3878942..3880057 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
JT729_RS18970 3880237..3880821 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
JT729_RS18975 3880818..3881186 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
JT729_RS18980 3881306..3881944 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
JT729_RS18985 3882209..3882496 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
JT729_RS18990 3882489..3882806 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JT729_RS18995 3882991..3884034 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
JT729_RS19000 3884704..3885570 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
JT729_RS19005 3885679..3887106 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T193995 WP_004142563.1 NZ_CP070430:c3882806-3882489 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T193995 NZ_CP093318:c14266-13916 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT193995 WP_004142561.1 NZ_CP070430:c3882496-3882209 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT193995 NZ_CP093318:c13911-13609 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References