Detailed information of TA system TA193913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2771..3429
• Replicon: plasmid pAB5075-VUB_3
• Accession: NZ_CP070365
• Organism: Acinetobacter baumannii strain AB5075-VUB

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JWB56_RS19360
• Protein ID: WP_000312250.1
• Coordinates: 3070..3429 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JWB56_RS19355
• Protein ID: WP_001096429.1
• Coordinates: 2771..3070 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JWB56_RS19335	189..818	+	630	WP_000701003.1	hypothetical protein	-
JWB56_RS19340	858..1076	+	219	WP_001043201.1	hypothetical protein	-
JWB56_RS19345	1096..1648	+	553	Protein_4	hypothetical protein	-
JWB56_RS19350	1698..2234	+	537	WP_000731978.1	hypothetical protein	-
JWB56_RS19355	2771..3070	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
JWB56_RS19360	3070..3429	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JWB56_RS19365	3629..4194	+	566	Protein_8	hypothetical protein	-
JWB56_RS19370	4243..4446	+	204	WP_205195844.1	hypothetical protein	-
JWB56_RS19375	5542..5832	+	291	Protein_10	hypothetical protein	-
JWB56_RS19380	5899..6285	+	387	Protein_11	hypothetical protein	-
JWB56_RS19385	6816..7223	+	408	Protein_12	Mu transposase C-terminal domain-containing protein	-
JWB56_RS19390	7226..8218	+	993	WP_001381192.1	TniB family NTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / dfrA7 / aac(6')-Ib / blaGES-11 / aph(6)-Id / aph(3'')-Ib / aadA2 / cmlA1 / ant(2'')-Ia / aph(3')-VIa	-	1..83362	83362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T193913 WP_000312250.1 NZ_CP070365:c3429-3070 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T193913 NZ_CP093280:c1072726-1071854 [Pseudomonas sp. T1-3-2]
ATGCACGCCACTGAAAGCCTCGACCCCTGTACCGAACTGCAAACCCTGTTGGCCAACCGCTTCGCCCAGCGCCTGCTGGA
CGTGGCTGGTGATCAGCAAGCCATTCGCGAAGCCGGCCGCGGCGCCGCCATCGTGCTGTTGCATGGCATCGGTTCGGGCG
CCGGCTCGTGGCTGGAAGTGGCGCTGCAACTTGGCCAGCACGCTCGGGTAGTTGCCTGGGATGCCCCGGGGTACGGCGAT
TCGACGCCGCTGAGGCCAGCGGCACCCCGTGCCGAGGACTACGCCGAACGCCTGCTCCACACCCTGGATGCACTGACCAT
CGAGCGTTGCGTGCTGGTCGGCCATTCGCTGGGGGCGATCACCGCCGCCGCGTTCAGCCGCCTGTACCCGCAACGCGTCA
GCCGCCTGGTGTTGCTGAGCCCCGCCCGCGGCTACGGCGACCCTGCCCGCGAGGTCCAGCGCCAGCAGGTCCGCGGCCAG
CGCCTGACAACACTGGAACAACTGGGCGTTGCGCAGATGGCTGCCCAACGCAGCAGCGCCATGCTCTCGCCGCGGCCCAC
CGCCAAGGCGTTGGCCTGGGTGCGCTGGAACATGGCACGGCTGAACCAACAAGGCTACCGCCAGGCCATCGAACTGCTGT
GCGGCGATGACCTGCTGCGCCATGCCCCGCTGCCCGTGCCATGCGAAGTGCACTGCGGCGAAGCCGATGCCATCACCGCG
CCCGAGACCTGCCTGACCTTGGCCAAGGCACTGGGCGCCAGCTTCAACCTGATCGCCGATGCAGGGCATGCCAGCCCCAT
CGAACAACCGCATGTCGTGGCCGGCCGCCTGGCCCATGCCCTCAAGGAATCCCTGACAGGTAGTGCACAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT193913 WP_001096429.1 NZ_CP070365:c3070-2771 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT193913 NZ_CP093280:c1071857-1071033 [Pseudomonas sp. T1-3-2]
ATGACCGACACCGATCTGACTAAAGATGCACAGGACAAATACATCGTTCCGGGCCTGGAGCGCGGCTTGTTGCTGCTGTG
CGAATTCAGCCGCCAGAACCGCACGCTGACTGCCCCGGAACTGGCTCGCCGCCTGGAACTGCCACGCTCGACCATCTTTC
GCCTGCTGACCACCCTGGAAACCATGGGCTTCGTGACCCGCAGCGGCAACGAATACCGCCTGGGCATGTCGGTACTGCGC
CTGGGCTTCGAGTACCTGGCTTCGCTGGAGCTGACCGAACTGGGCCAACCGCTGTTGGCGCGCTTGTGCGACAAGCTCAA
TTACCCCAGCAACCTGGTGGTGCGCGACGGCCGCTCGATTGTCTACGTAGCCAAGGTCTCGCCGCCGTCGGTGTTCTCCA
GCGCCGTCAATGTCGGCACACGCCTGCCCGCCCATGCCACGGTGCTGGGGCGCATTCTGCTGGAAGACTTGTCTTTGGCC
GAGCTGCGCGAGCTGTACCCTGAGGAGCACCTTGAACAGCACTCCCTGTGCACACCCAAGACCGTGCTGGAACTGTTCGA
CCTGGTGCAGGGCGACCGCCAGCGCGGCTATGTCAGCGGCGAGGGGTTCTTTGAATCGGCGATCTCCACCGTCGCCGCCC
CGGTACGCGACCACAGCGGCCGGATCGTCGCGGCGCTGGGTGTAACCATTCCGACCACCCAGATAGGCCACGTCAACTTC
GAGGAAACCCTGGCCCAGGTCCGTCGCAATGCCGACGAACTCTCGCGCTTGCTCAACTACACGCCCAGCCATGGCAGCCG
TGTCACCGCGCTGATGAGGGACTGA