Detailed information of TA system TA193823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1099789..1100306
• Replicon: chromosome
• Accession: NZ_CP070306
• Organism: Staphylococcus sp. SM9054

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: JQN77_RS05325
• Protein ID: WP_000113262.1
• Coordinates: 1100040..1100306 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: JQN77_RS05320
• Protein ID: WP_000584499.1
• Coordinates: 1099789..1100040 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQN77_RS05295	1095633..1096952	+	1320	WP_001808146.1	ISL3-like element IS1181 family transposase	-
JQN77_RS05300	1097031..1098281	-	1251	WP_001012223.1	FemA/FemB family glycyltransferase FmhA	-
JQN77_RS05305	1098536..1098898	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
JQN77_RS05310	1098917..1099516	+	600	WP_000162813.1	DsbA family protein	-
JQN77_RS05315	1099523..1099624	+	102	WP_031774922.1	hypothetical protein	-
JQN77_RS05320	1099789..1100040	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JQN77_RS05325	1100040..1100306	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
JQN77_RS05330	1100602..1102155	+	1554	WP_000727769.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
JQN77_RS05335	1102341..1102808	+	468	WP_001836792.1	SRPBCC domain-containing protein	-
JQN77_RS05340	1102895..1103077	+	183	WP_000079142.1	hypothetical protein	-
JQN77_RS05345	1103294..1104118	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
JQN77_RS05350	1104352..1104888	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1095633..1096952	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T193823 WP_000113262.1 NZ_CP070306:1100040-1100306 [Staphylococcus sp. SM9054]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T193823 NZ_CP093226:c3716349-3715951 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT193823 WP_000584499.1 NZ_CP070306:1099789-1100040 [Staphylococcus sp. SM9054]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT193823 NZ_CP093226:c3716645-3716352 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60