Detailed information of TA system TA193811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1102541..1103058
• Replicon: chromosome
• Accession: NZ_CP070305
• Organism: Staphylococcus sp. SM3655

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: JQN76_RS05345
• Protein ID: WP_000113262.1
• Coordinates: 1102792..1103058 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: JQN76_RS05340
• Protein ID: WP_000584499.1
• Coordinates: 1102541..1102792 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQN76_RS05315	1098385..1099704	+	1320	WP_001808146.1	ISL3-like element IS1181 family transposase	-
JQN76_RS05320	1099783..1101033	-	1251	WP_001012223.1	FemA/FemB family glycyltransferase FmhA	-
JQN76_RS05325	1101288..1101650	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
JQN76_RS05330	1101669..1102268	+	600	WP_000162813.1	DsbA family protein	-
JQN76_RS05335	1102275..1102376	+	102	WP_031774922.1	hypothetical protein	-
JQN76_RS05340	1102541..1102792	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JQN76_RS05345	1102792..1103058	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
JQN76_RS05350	1103354..1104907	+	1554	WP_000727769.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
JQN76_RS05355	1105093..1105560	+	468	WP_001836792.1	SRPBCC domain-containing protein	-
JQN76_RS05360	1105647..1105829	+	183	WP_000079142.1	hypothetical protein	-
JQN76_RS05365	1106046..1106870	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
JQN76_RS05370	1107104..1107640	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1098385..1099704	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T193811 WP_000113262.1 NZ_CP070305:1102792-1103058 [Staphylococcus sp. SM3655]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T193811 NZ_CP093226:c796367-795993 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT193811 WP_000584499.1 NZ_CP070305:1102541-1102792 [Staphylococcus sp. SM3655]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT193811 NZ_CP093226:c796825-796457 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60