Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2513876..2514438 Replicon chromosome
Accession NZ_CP070296
Organism Escherichia albertii strain Sample 165

Toxin (Protein)


Gene name higB Uniprot ID A0A7U8ZNU8
Locus tag JRC44_RS12355 Protein ID WP_000605679.1
Coordinates 2514160..2514438 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JRC44_RS12350 Protein ID WP_000781370.1
Coordinates 2513876..2514160 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC44_RS12335 (2509058) 2509058..2512105 + 3048 WP_077696004.1 formate dehydrogenase-N subunit alpha -
JRC44_RS12340 (2512118) 2512118..2513002 + 885 WP_001240583.1 formate dehydrogenase N subunit beta -
JRC44_RS12345 (2512995) 2512995..2513648 + 654 WP_000045636.1 formate dehydrogenase-N subunit gamma -
JRC44_RS12350 (2513876) 2513876..2514160 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JRC44_RS12355 (2514160) 2514160..2514438 - 279 WP_000605679.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JRC44_RS12360 (2514624) 2514624..2515634 - 1011 WP_000642385.1 alcohol dehydrogenase AdhP -
JRC44_RS12365 (2515769) 2515769..2517466 - 1698 WP_208629426.1 malate dehydrogenase -
JRC44_RS12370 (2517623) 2517623..2517760 - 138 WP_000841563.1 stationary-phase-induced ribosome-associated protein -
JRC44_RS12375 (2518027) 2518027..2518458 + 432 WP_000152289.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10533.10 Da        Isoelectric Point: 7.3995

>T193782 WP_000605679.1 NZ_CP070296:c2514438-2514160 [Escherichia albertii]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASSLVDIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T193782 NZ_CP093221:c651570-651397 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT193782 WP_000781370.1 NZ_CP070296:c2514160-2513876 [Escherichia albertii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT193782 NZ_CP093221:c651894-651598 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8ZNU8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References