Detailed information of TA system TA193773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1177417..1178144
• Replicon: chromosome
• Accession: NZ_CP070296
• Organism: Escherichia albertii strain Sample 165

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: JRC44_RS05705
• Protein ID: WP_000547563.1
• Coordinates: 1177417..1177728 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JRC44_RS05710
• Protein ID: WP_000126297.1
• Coordinates: 1177725..1178144 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC44_RS05675 (1172559)	1172559..1174268	+	1710	WP_025238503.1	formate hydrogenlyase subunit HycE	-
JRC44_RS05680 (1174278)	1174278..1174820	+	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
JRC44_RS05685 (1174820)	1174820..1175587	+	768	WP_025238504.1	formate hydrogenlyase subunit HycG	-
JRC44_RS05690 (1175584)	1175584..1175994	+	411	WP_001291913.1	formate hydrogenlyase assembly protein HycH	-
JRC44_RS05695 (1175987)	1175987..1176457	+	471	WP_000132954.1	hydrogenase maturation peptidase HycI	-
JRC44_RS05700 (1176500)	1176500..1177255	+	756	WP_025238505.1	hypothetical protein	-
JRC44_RS05705 (1177417)	1177417..1177728	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
JRC44_RS05710 (1177725)	1177725..1178144	+	420	WP_000126297.1	helix-turn-helix domain-containing protein	Antitoxin
JRC44_RS05715 (1178308)	1178308..1178664	-	357	WP_244251459.1	DUF4259 domain-containing protein	-
JRC44_RS05720 (1179087)	1179087..1179614	+	528	WP_001078780.1	electron transport protein HydN	-
JRC44_RS05725 (1179767)	1179767..1182019	+	2253	WP_025238507.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T193773 WP_000547563.1 NZ_CP070296:1177417-1177728 [Escherichia albertii]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T193773 NZ_CP093217:938060-938422 [Staphylococcus edaphicus]
ATGATGAGACGTGGCGACGTTTATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
TATTATACAGAATGATACTGGTAATAAATATAGTCCTACCGTAATTGTAGCTGCAATTACCGGTAGGATAAATAAAGCTA
AAATCCCTACACATGTGGAAATAGAAAAAAGTAAATATAAATTGGATAAAGATTCTGTTATATTGCTAGAGCAAATTAGG
ACTTTAGACAAGAATAGGCTGAAAGAAAAGTTAACGTACCTTTCTGACAAAAAAATGAAAGAAGTTAACAATGCCATTGA
TATAAGTTTAGGATTACATAATATTAGGGGTCATAAATCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15446.45 Da
• Isoelectric Point: 4.4596

>AT193773 WP_000126297.1 NZ_CP070296:1177725-1178144 [Escherichia albertii]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT193773 NZ_CP093217:937893-938063 [Staphylococcus edaphicus]
ATGGCAAGTTTCAACCAAAGTAGAAATCATAGTATAGAACAGCTATTAAAAGAAGGCTATTCTCAAATGGCTGACTTGAA
CCTCTCCCTTGCAGCAGAAGCGTTTCCATTTGAATGTGAAGCCTGTGATTGCAACGAAGTATATATCAGCTCCAAGAATA
ATAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22