Detailed information of TA system TA193768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 251902..252614
• Replicon: chromosome
• Accession: NZ_CP070296
• Organism: Escherichia albertii strain Sample 165

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: JRC44_RS01135
• Protein ID: WP_000162413.1
• Coordinates: 252312..252614 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JRC44_RS01130
• Protein ID: WP_000806446.1
• Coordinates: 251902..252240 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC44_RS01105 (246942)	246942..248924	+	1983	WP_208629588.1	TonB-dependent heme/hemoglobin receptor ChuA/ShuA	-
JRC44_RS01110 (248973)	248973..250001	+	1029	WP_001110408.1	hematinate-forming heme oxygenase ChuS	-
JRC44_RS01115 (250073)	250073..250603	-	531	WP_000480294.1	LuxR C-terminal-related transcriptional regulator	-
JRC44_RS01120 (250750)	250750..251316	-	567	WP_001044206.1	outer membrane lipoprotein Slp	-
JRC44_RS01125 (251678)	251678..251845	-	168	WP_232529606.1	hypothetical protein	-
JRC44_RS01130 (251902)	251902..252240	-	339	WP_000806446.1	HigA family addiction module antitoxin	Antitoxin
JRC44_RS01135 (252312)	252312..252614	-	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JRC44_RS01140 (252778)	252778..253257	+	480	WP_025238141.1	hypothetical protein	-
JRC44_RS01145 (253347)	253347..253520	-	174	WP_000553433.1	hypothetical protein	-
JRC44_RS01150 (253548)	253548..253631	+	84	WP_001295215.1	damage-inducible type I toxin DinQ	-
JRC44_RS01155 (253685)	253685..255037	-	1353	WP_054410173.1	glutathione-disulfide reductase	-
JRC44_RS01160 (255109)	255109..255951	-	843	WP_025238143.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T193768 WP_000162413.1 NZ_CP070296:c252614-252312 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T193768 NZ_CP093212:1185488-1185667 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT193768 WP_000806446.1 NZ_CP070296:c252240-251902 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT193768 NZ_CP093212:1184865-1185464 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7