Detailed information of TA system TA193757

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79217..79575
• Replicon: plasmid pESA139_1
• Accession: NZ_CP070293
• Organism: Escherichia albertii strain Sample 166

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JRC43_RS25715
• Protein ID: WP_233329041.1
• Coordinates: 79477..79575 (+)
• Length: 33 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	79217..79365 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC43_RS23925 (74753)	74753..75205	+	453	WP_086625668.1	single-stranded DNA-binding protein	-
JRC43_RS23930 (75263)	75263..75496	+	234	WP_000006017.1	DUF905 domain-containing protein	-
JRC43_RS23935 (75560)	75560..77518	+	1959	WP_208634882.1	ParB/RepB/Spo0J family partition protein	-
JRC43_RS23940 (77573)	77573..78007	+	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
JRC43_RS23945 (78004)	78004..78723	+	720	WP_089553879.1	plasmid SOS inhibition protein A	-
JRC43_RS23950 (78723)	78723..79241	+	519	WP_001178554.1	hypothetical protein	-
- (79217)	79217..79365	+	149	NuclAT_0	-	Antitoxin
- (79217)	79217..79365	+	149	NuclAT_0	-	Antitoxin
- (79217)	79217..79365	+	149	NuclAT_0	-	Antitoxin
- (79217)	79217..79365	+	149	NuclAT_0	-	Antitoxin
JRC43_RS25710 (79431)	79431..79508	+	78	Protein_96	DUF5431 family protein	-
JRC43_RS25715 (79477)	79477..79575	+	99	WP_233329041.1	Hok/Gef family protein	Toxin
JRC43_RS25720 (80037)	80037..80209	-	173	Protein_98	hypothetical protein	-
JRC43_RS25725 (80279)	80279..80485	+	207	WP_024204552.1	hypothetical protein	-
JRC43_RS23960 (80509)	80509..80805	+	297	WP_001470485.1	hypothetical protein	-
JRC43_RS23965 (80911)	80911..81102	+	192	Protein_101	DUF945 domain-containing protein	-
JRC43_RS23970 (81274)	81274..82473	-	1200	WP_000948429.1	IS91 family transposase	-
JRC43_RS23975 (82483)	82483..82671	-	189	WP_000957857.1	hypothetical protein	-
JRC43_RS23980 (82788)	82788..83427	+	640	Protein_104	DUF932 domain-containing protein	-
JRC43_RS23985 (83725)	83725..84294	-	570	Protein_105	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..107432	107432
	-	flank	IS/Tn	-	-	81274..82473	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-28)

Sequences

Toxin

Protein

• Length: 33 a.a.
• Molecular weight: 3808.36 Da
• Isoelectric Point: 7.9707

>T193757 WP_233329041.1 NZ_CP070293:79477-79575 [Escherichia albertii]
MFTYLTRKSLCEIRYRDGDREVAAFMAYESGK

Nucleotide

• Length: 99 bp

>T193757 NZ_CP093208:846875-847237 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Nucleotide

• Length: 149 bp

>AT193757 NZ_CP070293:79217-79365 [Escherichia albertii]
CAGGGTGCAGACATATGGGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGT
GTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCATCCCATATGTCT

Structures

Antitoxin