Detailed information of TA system TA193749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3718369..3719019
• Replicon: chromosome
• Accession: NZ_CP070292
• Organism: Escherichia albertii strain Sample 166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JRC43_RS18215
• Protein ID: WP_000263532.1
• Coordinates: 3718678..3719019 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3T5VD61
• Locus tag: JRC43_RS18210
• Protein ID: WP_025237546.1
• Coordinates: 3718369..3718668 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC43_RS18185 (3713601)	3713601..3713912	-	312	WP_000410252.1	hypothetical protein	-
JRC43_RS18190 (3713917)	3713917..3714309	-	393	WP_000273381.1	flagellar export chaperone FliS	-
JRC43_RS18195 (3714332)	3714332..3715648	-	1317	WP_000609691.1	flagellar filament capping protein FliD	-
JRC43_RS18200 (3716059)	3716059..3716973	-	915	WP_000949079.1	lateral flagellin LafA	-
JRC43_RS18205 (3717460)	3717460..3718311	+	852	WP_025237545.1	winged helix-turn-helix domain-containing protein	-
JRC43_RS18210 (3718369)	3718369..3718668	-	300	WP_025237546.1	XRE family transcriptional regulator	Antitoxin
JRC43_RS18215 (3718678)	3718678..3719019	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JRC43_RS18220 (3719095)	3719095..3720072	-	978	WP_025237547.1	hypothetical protein	-
JRC43_RS18225 (3720089)	3720089..3721018	-	930	WP_025237548.1	flagellar hook-associated protein FlgL	-
JRC43_RS18230 (3721033)	3721033..3722409	-	1377	WP_025237549.1	flagellar hook-associated protein FlgK	-
JRC43_RS18235 (3722613)	3722613..3722912	-	300	WP_025237550.1	rod-binding protein	-
JRC43_RS18240 (3722912)	3722912..3724012	-	1101	WP_025237551.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T193749 WP_000263532.1 NZ_CP070292:c3719019-3718678 [Escherichia albertii]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T193749 NZ_CP093202:1850588-1851049 [Staphylococcus epidermidis]
ATGGGGTTATATGAAAAAATGTTAATAAAACATGATTATATAGAAGTCAGAGAAACAAATGTTATGCCTAATGACCTACA
CGGTCTATGGTTAGGTGATTTAATTTTAATTAAGCGAAACCTTTCCGAAACACGTAAAGCTGAGGTATTATACGAAGAAC
TAACACACCATAAACTTACATATGGAAACATCTTAGACCAATCTAAATTCAACAACCGTAAATTTGAAAACTACGCTAGG
CGTTACGGGTATGAAGCTGCCCTACCTTTGCGCATTATTGTGGAGGCGCATAACTATGGTATTAGTAACTTATATGAACT
AGCTGAATATGTTCAATTAAGCGAAGAATATATAGTAGAAATATTGAAACATTACAAAAATAAATATGGTATTGGTACTC
ACTACGGAGAATACCTAATTACATTTGATCCGTTGAGGGTTTTTAAATATAAAGAAATATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10962.78 Da
• Isoelectric Point: 9.7665

>AT193749 WP_025237546.1 NZ_CP070292:c3718668-3718369 [Escherichia albertii]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRVAFQI

Nucleotide

• Length: 300 bp

>AT193749 NZ_CP093202:1850247-1850576 [Staphylococcus epidermidis]
ATGAGAAATAATGATGAAATAATCACAATAATTAAATCAGCTATGAAAGAACAAGATATGTCACTTAGTGAATTAGCTCG
TCGTGTAGGAGTTGCTAAATCTGCTGTATCACGTTATTTAAACTTAACTAGAGAATTTCCGTTAAATCGTACAGAAGATT
TTGCAAAAGCACTTAGTATCAGTACAGAATACTTACTTGGTTTTGACAAAAGTGAACAACAAGATGAACAACCAAAACAT
CGTGCAGCACATCTTGAAGGTGAATTAACAGATGAAGAATGGCAACGTGTGCTAGATTATGCAGATTATATAAGAAGCAA
ACGCAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3T5VD61