Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2535759..2536321 Replicon chromosome
Accession NZ_CP070292
Organism Escherichia albertii strain Sample 166

Toxin (Protein)


Gene name higB Uniprot ID A0A7U8ZNU8
Locus tag JRC43_RS12445 Protein ID WP_000605679.1
Coordinates 2535759..2536037 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JRC43_RS12450 Protein ID WP_000781370.1
Coordinates 2536037..2536321 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC43_RS12425 (2531739) 2531739..2532170 - 432 WP_000152289.1 peroxiredoxin OsmC -
JRC43_RS12430 (2532437) 2532437..2532574 + 138 WP_000841563.1 stationary-phase-induced ribosome-associated protein -
JRC43_RS12435 (2532731) 2532731..2534428 + 1698 WP_000433442.1 malate dehydrogenase -
JRC43_RS12440 (2534563) 2534563..2535573 + 1011 WP_000642385.1 alcohol dehydrogenase AdhP -
JRC43_RS12445 (2535759) 2535759..2536037 + 279 WP_000605679.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JRC43_RS12450 (2536037) 2536037..2536321 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JRC43_RS12455 (2536549) 2536549..2537202 - 654 WP_000045636.1 formate dehydrogenase-N subunit gamma -
JRC43_RS12460 (2537195) 2537195..2538079 - 885 WP_025237029.1 formate dehydrogenase N subunit beta -
JRC43_RS12465 (2538092) 2538092..2541139 - 3048 WP_077696004.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - nleC / vgrG/tssI 2383594..2638523 254929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10533.10 Da        Isoelectric Point: 7.3995

>T193747 WP_000605679.1 NZ_CP070292:2535759-2536037 [Escherichia albertii]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASSLVDIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T193747 NZ_CP093202:843957-844319 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT193747 WP_000781370.1 NZ_CP070292:2536037-2536321 [Escherichia albertii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT193747 NZ_CP093202:843790-843960 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8ZNU8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References