Detailed information of TA system TA193736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1196320..1197047
• Replicon: chromosome
• Accession: NZ_CP070292
• Organism: Escherichia albertii strain Sample 166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: JRC43_RS05810
• Protein ID: WP_000547563.1
• Coordinates: 1196320..1196631 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JRC43_RS05815
• Protein ID: WP_000126297.1
• Coordinates: 1196628..1197047 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC43_RS05780 (1191462)	1191462..1193171	+	1710	WP_208634829.1	formate hydrogenlyase subunit HycE	-
JRC43_RS05785 (1193181)	1193181..1193723	+	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
JRC43_RS05790 (1193723)	1193723..1194490	+	768	WP_025238504.1	formate hydrogenlyase subunit HycG	-
JRC43_RS05795 (1194487)	1194487..1194897	+	411	WP_001291913.1	formate hydrogenlyase assembly protein HycH	-
JRC43_RS05800 (1194890)	1194890..1195360	+	471	WP_000132954.1	hydrogenase maturation peptidase HycI	-
JRC43_RS05805 (1195403)	1195403..1196158	+	756	WP_025238505.1	hypothetical protein	-
JRC43_RS05810 (1196320)	1196320..1196631	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
JRC43_RS05815 (1196628)	1196628..1197047	+	420	WP_000126297.1	helix-turn-helix domain-containing protein	Antitoxin
JRC43_RS05820 (1197139)	1197139..1197567	-	429	WP_000536065.1	DUF4259 domain-containing protein	-
JRC43_RS05825 (1197990)	1197990..1198517	+	528	WP_001078780.1	electron transport protein HydN	-
JRC43_RS05830 (1198670)	1198670..1200922	+	2253	WP_208634830.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T193736 WP_000547563.1 NZ_CP070292:1196320-1196631 [Escherichia albertii]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T193736 NZ_CP093196:863752-863961 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15446.45 Da
• Isoelectric Point: 4.4596

>AT193736 WP_000126297.1 NZ_CP070292:1196628-1197047 [Escherichia albertii]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT193736 NZ_CP093196:863452-863745 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22