Detailed information of TA system TA193731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 253249..253961
• Replicon: chromosome
• Accession: NZ_CP070292
• Organism: Escherichia albertii strain Sample 166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: JRC43_RS01195
• Protein ID: WP_000162413.1
• Coordinates: 253659..253961 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JRC43_RS01190
• Protein ID: WP_000806446.1
• Coordinates: 253249..253587 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC43_RS01165 (248289)	248289..250272	+	1984	Protein_232	TonB-dependent heme/hemoglobin receptor ChuA/ShuA	-
JRC43_RS01170 (250321)	250321..251349	+	1029	WP_001110408.1	hematinate-forming heme oxygenase ChuS	-
JRC43_RS01175 (251421)	251421..251950	-	530	Protein_234	LuxR C-terminal-related transcriptional regulator	-
JRC43_RS01180 (252097)	252097..252663	-	567	WP_001044206.1	outer membrane lipoprotein Slp	-
JRC43_RS01185 (253025)	253025..253192	-	168	WP_232529606.1	hypothetical protein	-
JRC43_RS01190 (253249)	253249..253587	-	339	WP_000806446.1	HigA family addiction module antitoxin	Antitoxin
JRC43_RS01195 (253659)	253659..253961	-	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JRC43_RS01200 (254125)	254125..254604	+	480	WP_025238141.1	hypothetical protein	-
JRC43_RS01205 (254694)	254694..254867	-	174	WP_000553433.1	hypothetical protein	-
JRC43_RS01210 (254895)	254895..254978	+	84	WP_001295215.1	damage-inducible type I toxin DinQ	-
JRC43_RS01215 (255032)	255032..256384	-	1353	WP_054410173.1	glutathione-disulfide reductase	-
JRC43_RS01220 (256456)	256456..257298	-	843	WP_025238143.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T193731 WP_000162413.1 NZ_CP070292:c253961-253659 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T193731 NZ_CP093188:2033465-2033653 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT193731 WP_000806446.1 NZ_CP070292:c253587-253249 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT193731 NZ_CP093188:2033183-2033440 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7