Detailed information of TA system TA193696

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13966..14220
• Replicon: plasmid pESA118_1
• Accession: NZ_CP070288
• Organism: Escherichia albertii strain Sample 168

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: JRC45_RS23580
• Protein ID: WP_244259758.1
• Coordinates: 14071..14220 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	13966..14027 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC45_RS23555 (11146)	11146..11892	+	747	WP_208625715.1	conjugal transfer pilus acetylase TraX	-
JRC45_RS23560 (11947)	11947..12507	+	561	WP_171867720.1	fertility inhibition protein FinO	-
JRC45_RS23565 (12643)	12643..12855	+	213	WP_204629487.1	ANR family transcriptional regulator	-
JRC45_RS23570 (13092)	13092..13178	+	87	Protein_11	endonuclease	-
JRC45_RS23575 (13359)	13359..13670	-	312	WP_000765906.1	hypothetical protein	-
- (13966)	13966..14027	-	62	NuclAT_0	-	Antitoxin
- (13966)	13966..14027	-	62	NuclAT_0	-	Antitoxin
- (13966)	13966..14027	-	62	NuclAT_0	-	Antitoxin
- (13966)	13966..14027	-	62	NuclAT_0	-	Antitoxin
JRC45_RS23580 (14071)	14071..14220	+	150	WP_244259758.1	Hok/Gef family protein	Toxin
JRC45_RS23585 (14504)	14504..14761	+	258	WP_204629489.1	replication regulatory protein RepA	-
JRC45_RS23590 (14995)	14995..15069	+	75	WP_032319616.1	RepA leader peptide Tap	-
JRC45_RS23595 (15062)	15062..16084	+	1023	WP_204629490.1	plasmid replication initiator RepA	-
JRC45_RS23600 (16791)	16791..16949	+	159	WP_204629491.1	hypothetical protein	-
JRC45_RS23605 (17004)	17004..17273	+	270	WP_087905370.1	type II toxin-antitoxin system antitoxin YacA	-
JRC45_RS24705 (17273)	17273..17380	+	108	Protein_19	type II toxin-antitoxin system toxin YacB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espL2 / espL2	1..49739	49739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5499.64 Da
• Isoelectric Point: 8.2774

>T193696 WP_244259758.1 NZ_CP070288:14071-14220 [Escherichia albertii]
MTKYALIGLLAVCATVLCFSLIFRELLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T193696 NZ_CP093154:c4725716-4725345 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATTGATCTATTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGCCTGATCACATGGATGGAAGTCATGGTGGGTGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT193696 NZ_CP070288:c14027-13966 [Escherichia albertii]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin