Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 651223..651950 Replicon chromosome
Accession NZ_CP070287
Organism Escherichia albertii strain Sample 168

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JRC45_RS03225 Protein ID WP_000550189.1
Coordinates 651223..651537 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JRC45_RS03230 Protein ID WP_000560272.1
Coordinates 651534..651950 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRC45_RS03205 (647382) 647382..648368 - 987 WP_000617693.1 Gfo/Idh/MocA family oxidoreductase -
JRC45_RS03210 (648447) 648447..649139 - 693 WP_204629077.1 vancomycin high temperature exclusion protein -
JRC45_RS03215 (649216) 649216..649719 - 504 WP_204629078.1 M48 family metallopeptidase -
JRC45_RS03220 (649804) 649804..650940 + 1137 WP_059241776.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JRC45_RS03225 (651223) 651223..651537 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JRC45_RS03230 (651534) 651534..651950 + 417 WP_000560272.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JRC45_RS03235 (652040) 652040..654058 - 2019 WP_204629079.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JRC45_RS03240 (654284) 654284..656635 - 2352 WP_208625595.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T193675 WP_000550189.1 NZ_CP070287:651223-651537 [Escherichia albertii]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T193675 NZ_CP093151:327029-327397 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14993.40 Da        Isoelectric Point: 4.3697

>AT193675 WP_000560272.1 NZ_CP070287:651534-651950 [Escherichia albertii]
MIAIADILQAGEKLTDVAPFLAGIQSEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMVQAMPGG
VAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLNGKRKLTLEHAKKLAARFGISPALFID

Download         Length: 417 bp

>AT193675 NZ_CP093151:326811-327032 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References