Detailed information of TA system TA193673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 210309..211021
• Replicon: chromosome
• Accession: NZ_CP070287
• Organism: Escherichia albertii strain Sample 168

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: JRC45_RS00965
• Protein ID: WP_000162413.1
• Coordinates: 210309..210611 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JRC45_RS00970
• Protein ID: WP_000806446.1
• Coordinates: 210683..211021 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC45_RS00945 (206972)	206972..207814	+	843	WP_010326142.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-
JRC45_RS00950 (207886)	207886..209238	+	1353	WP_149541542.1	glutathione-disulfide reductase	-
JRC45_RS00955 (209292)	209292..209375	-	84	WP_001295215.1	damage-inducible type I toxin DinQ	-
JRC45_RS00960 (209666)	209666..210145	-	480	WP_204628992.1	hypothetical protein	-
JRC45_RS00965 (210309)	210309..210611	+	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JRC45_RS00970 (210683)	210683..211021	+	339	WP_000806446.1	HigA family addiction module antitoxin	Antitoxin
JRC45_RS00975 (211112)	211112..211261	+	150	WP_233991781.1	hypothetical protein	-
JRC45_RS00980 (211630)	211630..212196	+	567	WP_059216822.1	outer membrane lipoprotein Slp	-
JRC45_RS00985 (212343)	212343..212873	+	531	WP_000480294.1	LuxR C-terminal-related transcriptional regulator	-
JRC45_RS00990 (212945)	212945..213973	-	1029	WP_001110408.1	hematinate-forming heme oxygenase ChuS	-
JRC45_RS00995 (214022)	214022..216004	-	1983	WP_204628993.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T193673 WP_000162413.1 NZ_CP070287:210309-210611 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T193673 NZ_CP093150:541312-541662 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT193673 WP_000806446.1 NZ_CP070287:210683-211021 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT193673 NZ_CP093150:541026-541307 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7