Detailed information of TA system TA193603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 232561..233273
• Replicon: chromosome
• Accession: NZ_CP070281
• Organism: Escherichia albertii strain Sample 170

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: JRC42_RS01045
• Protein ID: WP_000162413.1
• Coordinates: 232971..233273 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JRC42_RS01040
• Protein ID: WP_000806446.1
• Coordinates: 232561..232899 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRC42_RS01015 (227662)	227662..229644	+	1983	WP_059239470.1	TonB-dependent heme/hemoglobin receptor ChuA/ShuA	-
JRC42_RS01020 (229693)	229693..230721	+	1029	WP_204619843.1	hemin-degrading factor	-
JRC42_RS01025 (230793)	230793..231323	-	531	WP_204619844.1	LuxR C-terminal-related transcriptional regulator	-
JRC42_RS01030 (231470)	231470..232036	-	567	WP_001044206.1	outer membrane lipoprotein Slp	-
JRC42_RS01035 (232325)	232325..232504	-	180	WP_059219034.1	hypothetical protein	-
JRC42_RS01040 (232561)	232561..232899	-	339	WP_000806446.1	HigA family addiction module antitoxin	Antitoxin
JRC42_RS01045 (232971)	232971..233273	-	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JRC42_RS01050 (233437)	233437..233916	+	480	WP_000532312.1	hypothetical protein	-
JRC42_RS01055 (234006)	234006..234179	-	174	WP_044708027.1	hypothetical protein	-
JRC42_RS01060 (234207)	234207..234290	+	84	WP_001295215.1	damage-inducible type I toxin DinQ	-
JRC42_RS01065 (234344)	234344..235696	-	1353	WP_054410173.1	glutathione-disulfide reductase	-
JRC42_RS01070 (235768)	235768..236610	-	843	WP_000954227.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T193603 WP_000162413.1 NZ_CP070281:c233273-232971 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T193603 NZ_CP093037:c916383-916099 [Bartonella krasnovii]
ATGAAAATAACCCCATTTAAACCAGAAGAATATCTTAAAGACATTGAGTCACAGAAAATTTTTCTCAATGAAGCCCTTAA
AACCGGTGATGCCGCCCACATTGCCGATGCTCTTGGTATCGTTGCTCGTGCCCAAAATATGAGTGCCTTAGCAAAAGAAA
CCAATCGCGAACGCAGTGGATTATACCGTTCCTTAAGTAAAACGGGTGACCCAAAGCTTTCTACCTTAGTCGCTGTATTA
TCTGCCCTTAATTTACAACTCTCTGTACAATCTTCTACTTCATAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT193603 WP_000806446.1 NZ_CP070281:c232899-232561 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT193603 NZ_CP093037:c916672-916364 [Bartonella krasnovii]
ATGATACTCATTCATAAAACAATAGAATTTGATACTTGGCTTAAGAAACTTAAAGATAAAAGCGTTAAGGCTATTATTCT
TCAACGTGTTGTACGCTTAAAACAAGGACTTTTCGGTGATGTTAAATTCTTTCATGGCATAGGGGAATTACGTATCCACT
ATGGTGCTGGTTACAGAATTTATTTTACCCAAAAAGGCTCTGATTTTATCCTTTTGTTATGCGGTGGAGATAAATCAACG
CAGCAAAGAGATATTGAACAAGCGTTAAAATTAAAAGAGGAGTACAGTGATGAAAATAACCCCATTTAA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7