193594

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3623305..3623999 Replicon chromosome
Accession NZ_CP070232
Organism Escherichia coli strain 10R

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JTF63_RS17385 Protein ID WP_001263489.1
Coordinates 3623305..3623703 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JTF63_RS17390 Protein ID WP_000554758.1
Coordinates 3623706..3623999 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3618893..3618973 - 81 NuclAT_11 - -
- 3618893..3618973 - 81 NuclAT_11 - -
- 3618893..3618973 - 81 NuclAT_11 - -
- 3618893..3618973 - 81 NuclAT_11 - -
JTF63_RS17360 3619569..3620027 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JTF63_RS17365 3620288..3621745 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JTF63_RS17370 3621802..3622323 - 522 Protein_3394 peptide chain release factor H -
JTF63_RS17375 3622319..3622525 - 207 Protein_3395 RtcB family protein -
JTF63_RS17380 3622843..3623295 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
JTF63_RS17385 3623305..3623703 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JTF63_RS17390 3623706..3623999 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JTF63_RS17395 3624051..3625106 - 1056 WP_001226164.1 DNA polymerase IV -
JTF63_RS17400 3625177..3625962 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JTF63_RS17405 3625934..3627646 + 1713 Protein_3401 flagellar biosynthesis protein FlhA -
JTF63_RS17410 3627870..3628367 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3623305..3638584 15279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T193594 WP_001263489.1 NZ_CP070232:c3623703-3623305 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T193594 NZ_CP093035:c832351-832067 [Bartonella krasnovii]
ATGAAAATAACCCCATTTAAACCAGAAGAATATCTTAAAGACATTGAGTCACAGAAAATTTTTCTCAATGAAGCCCTTAA
AACCGGTGATGCCGCCCACATTGCCGATGCTCTTGGTATCGTTGCTCGTGCCCAAAATATGAGTGCCTTAGCAAAAGAAA
CCAATCGCGAACGCAGTGGATTATACCGTTCCTTAAGTAAAACGGGTGACCCAAAGCTTTCTACCTTAGTCGCTGTATTA
TCTGCCCTTAATTTACAACTCTCTGTACAATCTTCTACTTCATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT193594 WP_000554758.1 NZ_CP070232:c3623999-3623706 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT193594 NZ_CP093035:c832640-832332 [Bartonella krasnovii]
ATGATACTCATTCATAAAACAATAGAATTTGATACTTGGCTTAAGAAACTTAAAGATAAAAGCGTTAAGGCTATTATTCT
TCAACGTGTTGTACGCTTAAAACAAGGACTTTTCGGTGATGTTAAATTCTTTCATGGCATAGGGGAATTACGTATCCACT
ATGGTGCTGGTTACAGAATTTATTTTACCCAAAAAGGCTCTGATTTTATCCTTTTGTTATGCGGTGGAGATAAATCAACG
CAGCAAAGAGATATTGAACAAGCGTTAAAATTAAAAGAGGAGTACAGTGATGAAAATAACCCCATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References