Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4870922..4871424 Replicon chromosome
Accession NZ_CP070229
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JTC67_RS24240 Protein ID WP_000767829.1
Coordinates 4871170..4871424 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JTC67_RS24235 Protein ID WP_001259255.1
Coordinates 4870922..4871173 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTC67_RS24210 (4866100) 4866100..4867167 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JTC67_RS24215 (4867167) 4867167..4868237 - 1071 WP_053894041.1 histidinol-phosphate transaminase -
JTC67_RS24220 (4868234) 4868234..4869538 - 1305 WP_032344119.1 histidinol dehydrogenase -
JTC67_RS24225 (4869544) 4869544..4870443 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JTC67_RS24230 (4870589) 4870589..4870639 - 51 WP_001364200.1 his operon leader peptide -
JTC67_RS24235 (4870922) 4870922..4871173 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JTC67_RS24240 (4871170) 4871170..4871424 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JTC67_RS24245 (4871507) 4871507..4872331 + 825 WP_021524296.1 SDR family oxidoreductase -
JTC67_RS24250 (4872377) 4872377..4873306 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
JTC67_RS24255 (4873521) 4873521..4873583 + 63 WP_010723108.1 membrane protein YoeI -
JTC67_RS24260 (4873573) 4873573..4874931 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T193565 WP_000767829.1 NZ_CP070229:4871170-4871424 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T193565 NZ_CP093029:c60327-59977 [Pseudomonas aeruginosa]
ATGAGCAAACGCCTGCAGCATCAGTTCGACCGCGGCGATATCGTCTCCTTGGACATGGGTAGCGCTTCAGCACAGGCGGC
CTGCAAAGCGCTCGTGCTTTCCCCTGCAGCGTACAACGTCCTGGGGCTCGCCCTAGCCATTCCTATCACCGAGCACGACA
GCAGCCGCTACGCCGGCTTTGCCGTCGCGATCCTCCTTCCAGGGCGCACGCCGGCGAAGGGGGCAGCCTTGGTCAACCTG
GTGCGGCCGGTGGATCTTGCTGCAAGAGGTGCAAAGCTGCTCGGGAAGGCGCCCCAGACGACCATAGATGAAGCCCTGCA
GCGCCTGCAGGCGGTTGTTGGGAAAGACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT193565 WP_001259255.1 NZ_CP070229:4870922-4871173 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT193565 NZ_CP093029:c60572-60324 [Pseudomonas aeruginosa]
TTGAAAACGACTATCCGTGCGATTGGGAACTCTCAGGGCATCACGATACCCAAGCCGTTGCTGGCCGAGCTCGGCGTAAG
CCTTGGCGACCCTGTAGACATCTCCTTCAAAGACCAGACCTTGGTGGTGACCAAGGTCGCCACCGAATATGACCTCAGCA
CGCTAGTCGCCCAGTGTGACCTGGAGGCCCCGGAGCCGCCGGACATCCAATGCTGGAGAACAGCTACGCCGGTAGGAAAC
GAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References