Detailed information of TA system TA193564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4101769..4102496
• Replicon: chromosome
• Accession: NZ_CP070229
• Organism: Escherichia coli strain EC12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: JTC67_RS20655
• Protein ID: WP_000547563.1
• Coordinates: 4101769..4102080 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JTC67_RS20660
• Protein ID: WP_000126304.1
• Coordinates: 4102077..4102496 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTC67_RS20625 (4096913)	4096913..4098622	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
JTC67_RS20630 (4098632)	4098632..4099174	+	543	WP_000493787.1	formate hydrogenlyase subunit HycF	-
JTC67_RS20635 (4099174)	4099174..4099941	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
JTC67_RS20640 (4099938)	4099938..4100348	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
JTC67_RS20645 (4100341)	4100341..4100808	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
JTC67_RS20650 (4100851)	4100851..4101606	+	756	WP_001374644.1	hypothetical protein	-
JTC67_RS20655 (4101769)	4101769..4102080	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
JTC67_RS20660 (4102077)	4102077..4102496	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
JTC67_RS20665 (4102588)	4102588..4103016	-	429	WP_000536064.1	hypothetical protein	-
JTC67_RS20670 (4103401)	4103401..4103928	+	528	WP_001078777.1	electron transport protein HydN	-
JTC67_RS20675 (4104081)	4104081..4106333	+	2253	WP_001592558.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T193564 WP_000547563.1 NZ_CP070229:4101769-4102080 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T193564 NZ_CP093029:57719-58000 [Pseudomonas aeruginosa]
ATGCGGACGATTGAACAGACCAGCCGGTTCAAGCGCGATTACAAACGCGAAGCCAAAGGCCCGCATCGTCAGACGCTGGC
GAGCGATTTCGTCGCCATTGTCACAGCCCTTGCGAATGACCAGCCGCTGGCCGAGAAGCACCGCGACCACGCGCTCAATG
GCGACTGGAAAGATCACCGGGACTGCCACATCAAGCCTGACCTGGTGCTGATTTACCGCAAGCCGGATGACGCCGTGTTG
CAACTCGTGCGTCTCGGCTCGCATAGCGAGCTTGGCCTGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT193564 WP_000126304.1 NZ_CP070229:4102077-4102496 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT193564 NZ_CP093029:57469-57732 [Pseudomonas aeruginosa]
ATGTCTGCCAATGCTGTAGTCCGTGCCCGTATCGACGAGCACATCAAGGAAGAGGCGACCGTCGTGCTGGCGGCGATGGG
GCTCACCGTGTCCGACGCCTTCCGCATCATGTTGACCCGCGTCGCCCGCGAGAAAGCCTTGCCGTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCGCGCCGTGGTGGCCTGAAGTCGTTTGCTTCCGTCGAGGACCTGATG
GCGGACCTGAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22