Detailed information of TA system TA193558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 3193189..3194507
• Replicon: chromosome
• Accession: NZ_CP070229
• Organism: Escherichia coli strain EC12

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: JTC67_RS16210
• Protein ID: WP_062860100.1
• Coordinates: 3193500..3194507 (+)
• Length: 336 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2A7M399
• Locus tag: JTC67_RS16205
• Protein ID: WP_001490601.1
• Coordinates: 3193189..3193500 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTC67_RS16180 (3188721)	3188721..3189740	+	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
JTC67_RS16185 (3189750)	3189750..3190652	+	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
JTC67_RS16190 (3190663)	3190663..3191646	+	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	-
JTC67_RS16195 (3191643)	3191643..3192656	+	1014	WP_001357946.1	dipeptide ABC transporter ATP-binding subunit DppF	-
JTC67_RS16200 (3192881)	3192881..3193204	+	324	WP_021560081.1	helix-turn-helix transcriptional regulator	-
JTC67_RS16205 (3193189)	3193189..3193500	+	312	WP_001490601.1	HipA N-terminal domain-containing protein	Antitoxin
JTC67_RS16210 (3193500)	3193500..3194507	+	1008	WP_062860100.1	HipA domain-containing protein	Toxin
JTC67_RS16215 (3194524)	3194524..3195795	-	1272	WP_001312176.1	amino acid permease	-
- (3196157)	3196157..3196222	-	66	NuclAT_25	-	-
- (3196157)	3196157..3196222	-	66	NuclAT_25	-	-
- (3196157)	3196157..3196222	-	66	NuclAT_25	-	-
- (3196157)	3196157..3196222	-	66	NuclAT_25	-	-
JTC67_RS16220 (3196271)	3196271..3196378	+	108	WP_000170747.1	type I toxin-antitoxin system toxin Ldr family protein	-
- (3196640)	3196640..3196705	-	66	NuclAT_24	-	-
- (3196640)	3196640..3196705	-	66	NuclAT_24	-	-
- (3196640)	3196640..3196705	-	66	NuclAT_24	-	-
- (3196640)	3196640..3196705	-	66	NuclAT_24	-	-
JTC67_RS16225 (3196754)	3196754..3196861	+	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
JTC67_RS16230 (3196947)	3196947..3198626	-	1680	WP_021560079.1	cellulose biosynthesis protein BcsG	-
JTC67_RS16235 (3198623)	3198623..3198814	-	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-302)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 336 a.a.
• Molecular weight: 38453.69 Da
• Isoelectric Point: 5.2501

>T193558 WP_062860100.1 NZ_CP070229:3193500-3194507 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDYKGLPVHQEQLDGAMQITD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPTLAPVYDFVSVAPY
PEYFYSNYLELPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Nucleotide

• Length: 1008 bp

>T193558 NZ_CP093028:2440709-2441284 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11822.40 Da
• Isoelectric Point: 6.0609

>AT193558 WP_001490601.1 NZ_CP070229:3193189-3193500 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAQDYHGPAISISLPVAQRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Nucleotide

• Length: 312 bp

>AT193558 NZ_CP093028:2440242-2440712 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2A7M399