Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 582759..583321 Replicon chromosome
Accession NZ_CP070229
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag JTC67_RS03210 Protein ID WP_000605675.1
Coordinates 582759..583037 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JTC67_RS03215 Protein ID WP_000781370.1
Coordinates 583037..583321 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTC67_RS03185 (578345) 578345..578776 - 432 WP_000152305.1 peroxiredoxin OsmC -
JTC67_RS03190 (579121) 579121..579336 + 216 WP_000495766.1 biofilm-dependent modulation protein -
JTC67_RS03195 (579438) 579438..579575 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JTC67_RS03200 (579732) 579732..581429 + 1698 WP_000433460.1 malate dehydrogenase -
JTC67_RS03205 (581563) 581563..582573 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
JTC67_RS03210 (582759) 582759..583037 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JTC67_RS03215 (583037) 583037..583321 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JTC67_RS03220 (583550) 583550..584203 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
JTC67_RS03225 (584196) 584196..585080 - 885 WP_062860068.1 formate dehydrogenase N subunit beta -
JTC67_RS03230 (585093) 585093..588140 - 3048 WP_077818373.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T193549 WP_000605675.1 NZ_CP070229:582759-583037 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T193549 NZ_CP093022:c5164124-5163777 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT193549 WP_000781370.1 NZ_CP070229:583037-583321 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT193549 NZ_CP093022:c5164385-5164134 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References