Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4388876..4389675 Replicon chromosome
Accession NZ_CP070227
Organism Escherichia coli strain JA0072

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag JS570_RS20775 Protein ID WP_000347251.1
Coordinates 4389211..4389675 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag JS570_RS20770 Protein ID WP_001296435.1
Coordinates 4388876..4389211 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JS570_RS20755 (4384661) 4384661..4385431 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
JS570_RS20760 (4385447) 4385447..4386781 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
JS570_RS20765 (4387156) 4387156..4388727 + 1572 WP_001273939.1 galactarate dehydratase -
JS570_RS20770 (4388876) 4388876..4389211 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JS570_RS20775 (4389211) 4389211..4389675 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JS570_RS20780 (4389730) 4389730..4390539 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JS570_RS20785 (4390788) 4390788..4392068 + 1281 WP_000681930.1 tagatose-bisphosphate aldolase subunit KbaZ -
JS570_RS20790 (4392091) 4392091..4392564 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
JS570_RS20795 (4392575) 4392575..4393354 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JS570_RS20800 (4393344) 4393344..4394222 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JS570_RS20805 (4394240) 4394240..4394674 + 435 WP_000948834.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T193538 WP_000347251.1 NZ_CP070227:4389211-4389675 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T193538 NZ_CP093021:c139403-139122 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTATGACCATTACGTCGTGCTGATCGGCCCGGA
GAAAGCGCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAAGAGGTGGCCAACCAGGGGGCGGGGCGAC
CCAGCGAGGTGCCAGGCGTACGCACCCTGGCGCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTAGAAATTACCCCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT193538 WP_001296435.1 NZ_CP070227:4388876-4389211 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT193538 NZ_CP093021:c139627-139400 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References