Detailed information of TA system TA193499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2289949..2290546
• Replicon: chromosome
• Accession: NZ_CP070172
• Organism: Klebsiella pneumoniae strain FDAARGOS_1315

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: I6K45_RS11225
• Protein ID: WP_004893639.1
• Coordinates: 2289949..2290266 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: I6K45_RS11230
• Protein ID: WP_004142561.1
• Coordinates: 2290259..2290546 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K45_RS11210	2285653..2287080	-	1428	WP_009308097.1	MFS transporter	-
I6K45_RS11215	2287189..2288055	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
I6K45_RS11220	2288721..2289764	+	1044	WP_032410657.1	DUF2157 domain-containing protein	-
I6K45_RS11225	2289949..2290266	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K45_RS11230	2290259..2290546	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
I6K45_RS11235	2290811..2291464	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
I6K45_RS11240	2291584..2291952	-	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
I6K45_RS11245	2291952..2292533	-	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
I6K45_RS11250	2292713..2293828	+	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
I6K45_RS11255	2293859..2294200	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
I6K45_RS11260	2294218..2294466	-	249	WP_032410665.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T193499 WP_004893639.1 NZ_CP070172:2289949-2290266 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T193499 NZ_CP093011:4978266-4978640 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT193499 WP_004142561.1 NZ_CP070172:2290259-2290546 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT193499 NZ_CP093011:4977809-4978177 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1