Detailed information of TA system TA193485

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86547..86876
• Replicon: plasmid unnamed
• Accession: NZ_CP070171
• Organism: Escherichia coli strain FDAARGOS_1284

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K14_RS24080
• Protein ID: WP_001372321.1
• Coordinates: 86547..86672 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	86749..86876 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K14_RS24040 (81657)	81657..81884	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
I6K14_RS24045 (81972)	81972..82649	-	678	WP_204653784.1	PAS domain-containing protein	-
I6K14_RS24050 (82783)	82783..83166	-	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K14_RS24055 (83509)	83509..84099	+	591	WP_277996679.1	transglycosylase SLT domain-containing protein	-
I6K14_RS24060 (84396)	84396..85217	-	822	WP_001234469.1	DUF932 domain-containing protein	-
I6K14_RS24065 (85338)	85338..85625	-	288	WP_000107535.1	hypothetical protein	-
I6K14_RS24070 (85650)	85650..85856	-	207	WP_000275859.1	hypothetical protein	-
I6K14_RS24690 (85926)	85926..86099	+	174	Protein_106	hypothetical protein	-
I6K14_RS24695 (86097)	86097..86327	-	231	WP_001426396.1	hypothetical protein	-
I6K14_RS24080 (86547)	86547..86672	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K14_RS24700 (86614)	86614..86763	-	150	Protein_109	plasmid maintenance protein Mok	-
- (86749)	86749..86876	-	128	NuclAT_0	-	Antitoxin
- (86749)	86749..86876	-	128	NuclAT_0	-	Antitoxin
- (86749)	86749..86876	-	128	NuclAT_0	-	Antitoxin
- (86749)	86749..86876	-	128	NuclAT_0	-	Antitoxin
- (88318)	88318..88420	-	103	NuclAT_1	-	-
- (88318)	88318..88420	-	103	NuclAT_1	-	-
- (88318)	88318..88420	-	103	NuclAT_1	-	-
- (88318)	88318..88420	-	103	NuclAT_1	-	-
I6K14_RS24090 (88389)	88389..89151	-	763	Protein_111	plasmid SOS inhibition protein A	-
I6K14_RS24095 (89148)	89148..89582	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
I6K14_RS24100 (89637)	89637..91595	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catA1 / dfrA8 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD	-	1..118581	118581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T193485 WP_001372321.1 NZ_CP070171:c86672-86547 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T193485 NZ_CP093011:c2243549-2243235 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Nucleotide

• Length: 128 bp

>AT193485 NZ_CP070171:c86876-86749 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin